Gene therapy of experimental malignant mesothelioma using adenovirus vectors encoding the HSVtk gene

Gene Therapy
M C EsandiJ L Noteboom

Abstract

Replication-defective adenovirus vectors were generated in which the gene of interest (lacZ, luciferase or HSV-tk) is driven by the adenovirus major late promoter (MLP) or the human cytomegalovirus immediate-early gene promoter/enhancer (CMV). In vitro experiments with rat (II-45) and human (MERO 25) mesothelioma cell lines revealed that the CMV promoter was stronger than the MLP promoter regarding levels of expression of the luciferase reporter gene and ganciclovir (GCV) killing efficiency after tk gene transfer. Following administration of IG.Ad.CMV.lacZ recombinant adenovirus (Introgene, IG) into the pleural cavity of Fischer rats with established mesothelioma, a widespread distribution of infectious virus particles through the thorax contents was demonstrated. However, a relatively small proportion of tumor cells were transduced. Nevertheless, a strong tumor growth inhibition was observed following treatment with IG.Ad.CMV.TK recombinant adenovirus and GCV. Separate groups of rats inoculated on day 0 with 10(5) II-45 cells in the pleural cavity, received 7 x 10(9) infectious particles of IG.Ad. CMV.TK on day 1, day 2, day 4 or day 8. One day after virus administration, 25 mg/kg GCV or PBS (controls) was injected i.p. (intra...Continue Reading

Citations

Oct 8, 1999·Pharmacology & Therapeutics·G M Dubowchik, M A Walker
Jan 28, 2006·Cancer Gene Therapy·R G van der MostD J Nelson
Oct 19, 2010·Avian Pathology : Journal of the W.V.P.A·Sameer A GreenallSandra I Sapats
Feb 27, 1999·Human Gene Therapy·M Y HurwitzR L Hurwitz
Feb 10, 2000·Annals of the New York Academy of Sciences·D T Curiel
Feb 23, 2000·Journal of Virology·I GanlyA Balmain
Jul 30, 2005·Expert Opinion on Biological Therapy·Delia J NelsonRobbert van der Most
Sep 3, 2013·Clinics in Chest Medicine·Andrew R Haas, Daniel H Sterman
Dec 6, 2005·Hematology/oncology Clinics of North America·Daniel H Sterman
Jun 24, 2006·Journal of Controlled Release : Official Journal of the Controlled Release Society·Takehiro SerikawaKenichi Tanaka
Apr 9, 2004·International Journal of Occupational and Environmental Health·Duncan J StewartKenneth J O'Byrne
Jul 31, 1998·Hematology/oncology Clinics of North America·D H StermanS M Albelda
Aug 15, 2002·BMC Molecular Biology·Pierre LefesvreDirk van Bekkum
May 30, 2001·Japanese Journal of Cancer Research : Gann·S AkasakaT Shimada
Dec 9, 2016·Human Gene Therapy·Dolan SondhiRonald G Crystal
Oct 7, 2017·Future Oncology·Navid SobhaniDaniele Generali
Aug 6, 2013·Journal of Animal Physiology and Animal Nutrition·F Péron, C Grosset
Oct 26, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Daniel H StermanSteven M Albelda

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