Gene therapy of rat malignant gliomas using neural stem cells expressing IL-12

DNA and Cell Biology
Shu-Yuan YangJian-Ning Zhang

Abstract

Primary malignant brain tumors have a poor prognosis. This report investigates the potential for gene therapy of experimental brain tumors using neural stem cells (NSCs) expressing IL-12. In this study NSCs were isolated from the hippocampi of 3-5-month human embryos and used for lipofectamine mediated transfer of the IL-12 gene. Positive clones of anti-G418 were obtained and were proliferated in culture and expression of IL-12 was demonstrated by RT-PCR. For the in vivo studies three groups of rats were used and stereotactic injections were made into the striatum. In the first group C6 tumor cells were injected, in the second C6 cells and hNSCs. IL-12, and in the third C6 cells on Day 0 followed by hNSCs.IL-12 on day 5. The growth of the resulting tumors was monitored by magnetic resonance imaging (MRI) and after sacrifice by immunohistochemistry. Rats injected with C6 cells and hNSCs.IL-12 had a significantly prolonged survival. Injections of hNSCs.IL-12 were also made into established gliomas. The survival time was also significantly prolonged compared to controls. MR imaging demonstrated that there was initial growth of tumor followed by shrinkage and then disappearance. After sacrifice, tumor areas were studied by histoche...Continue Reading

References

Aug 1, 1992·Neurosurgery·D KondziolkaJ C Flickinger
Aug 1, 1992·Cellular Immunology·M K GatelyR Chizzonite
Oct 1, 1991·Immunology Today·T RoszmanW Brooks
Sep 1, 1993·Journal of Neuro-oncology·M Tada, N de Tribolet
Jun 1, 1994·Experimental Neurology·R AntonR H Edwards
Jan 1, 1995·Experimental Brain Research·C N SvendsenS B Dunnett
Jan 1, 1995·European Journal of Immunology·S GillessenM K Gately
Sep 1, 1993·European Journal of Immunology·F MattnerT Germann
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·F H GageJ Ray
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·A L RakhmilevichN S Yang
Jun 25, 1996·Proceedings of the National Academy of Sciences of the United States of America·A Martínez-SerranoA Björklund
Oct 31, 1996·Annals of the New York Academy of Sciences·A L AngiolilloG Tosato
Jan 1, 1997·Journal of the National Cancer Institute·J A Roth, R J Cristiano
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·H N LodeR A Reisfeld
May 19, 1998·Nature Medicine·D M Pardoll
Jan 5, 1999·Journal of Neuroscience Methods·C N SvendsenM A Caldwell
Jul 23, 1999·Experimental Neurology·M K CarpenterL U Wahlberg
Dec 10, 1999·Experimental Cell Research·C B JohanssonJ Frisén
Feb 19, 2000·Proceedings of the National Academy of Sciences of the United States of America·J N ParkerJ M Markert
Mar 4, 2000·Journal of Neurosurgery·F DiMecoA Olivi
Mar 31, 2000·Nature Medicine·S BenedettiG Finocchiaro
Jul 7, 2000·Molecular and Cellular Neurosciences·F J RubioA Martínez-Serrano
Oct 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·D BalickiH N Lode

❮ Previous
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Citations

May 4, 2011·Stem Cell Reviews and Reports·Kristien ReekmansPeter Ponsaerts
Dec 8, 2007·Neuroscience Bulletin·Feng Xu, Jian-Hong Zhu
Jun 30, 2009·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Michael C Oh, Daniel A Lim
Jul 27, 2012·Therapeutic Delivery·Carmen Gutiérrez MillánJosé M Lanao
Jul 20, 2011·Journal of Microencapsulation·Kerstin KleinschmidtThomas Brinker
Apr 21, 2006·Expert Review of Neurotherapeutics·Maciej S Lesniak
Mar 7, 2012·Expert Review of Clinical Pharmacology·Mamta WankhedeCostas G Hadjipanayis
Aug 22, 2012·Neuroscience Letters·NaTosha N GatsonBalveen Kaur
Jul 4, 2012·Neurosurgery Clinics of North America·Edjah K NduomCostas G Hadjipanayis
Mar 26, 2009·Methods : a Companion to Methods in Enzymology·Yannic WaerzeggersAndreas H Jacobs
Mar 21, 2009·Journal of Neuroscience Research·Seung U Kim, Jean de Vellis
Aug 5, 2009·International Journal of Cancer. Journal International Du Cancer·Javier MercapideAurelio Lorico
May 16, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Stephen YipEvan Y Snyder
Oct 4, 2005·Neurological Research·Christian W Sikorski, Maciej S Lesniak
Apr 1, 2008·The Lancet Oncology·Maarten F Corsten, Khalid Shah
Sep 15, 2017·American Journal of Clinical Oncology·Elizabeth ReznikA Gabriella Wernicke
Aug 24, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Seung-Ki KimRona S Carroll
Sep 25, 2020·Hematology/oncology and Stem Cell Therapy·Banafshe AbadiCristian Vergallo
Apr 2, 2021·Frontiers in Molecular Neuroscience·Kaushik BanerjeeMaria G Castro
May 20, 2021·CNS Oncology·Anda-Alexandra CalinescuSue K O'Shea

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