Gene therapy targeting leiomyoma: adenovirus-mediated delivery of dominant-negative estrogen receptor gene shrinks uterine tumors in Eker rat model.

Fertility and Sterility
M H HassanAyman Al-Hendy

Abstract

To evaluate the utility of gene therapy for uterine fibroids in the Eker rat model using an adenovirus-mediated delivery of a dominant-negative estrogen receptor gene (Ad-DNER). Animal study. University animal laboratory. Twenty-seven female Eker rats. We randomized Eker rats with magnetic resonance imaging (MRI)-confirmed uterine leiomyomas to a single treatment of direct intrafibroid injection with Ad-DNER, Ad-bacterial ss-galactosidase, or vehicle. Tumor volumes were determined by MRI scanning and caliper measurement. Samples of serum, fibroid tumors, and various organs were collected at 8, 15, and 30 days after treatment to assess treatment safety and efficacy. The Ad-DNER treatment significantly decreased uterine fibroid volume by 45%, 80%, and 77.4% of pretreatment volume at days 8, 15, and 30, respectively, and modulated the expression of apoptosis-, proliferation-, and extracellular matrix-related genes' compared with control animals. The Ad-DNER did not produce any toxic effects in nontarget tissues. The Ad-DNER treatment shrinks Eker rats' fibroids, in part, via modulation of several estrogen-regulated genes. This safe gene therapy approach presents a promising conservative treatment option for women with symptomatic ...Continue Reading

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Citations

Feb 4, 2012·Biology of Reproduction·Sunil K HalderAyman Al-Hendy
Oct 1, 2013·Human Reproduction Update·Reshef Tal, James H Segars
Jan 23, 2016·Best Practice & Research. Clinical Obstetrics & Gynaecology·Banafsheh N KashaniFelice Petraglia
Jun 16, 2011·Molecular and Cellular Endocrinology·J Julie Kim, Elizabeth C Sefton
Apr 6, 2013·Fertility and Sterility·Md Soriful IslamPasquapina Ciarmela
Aug 26, 2010·Fertility and Sterility·Chakradhari SharanAyman Al-Hendy
Feb 13, 2016·Reproductive Sciences·Essam R OthmanAyman Al-Hendy
May 14, 2014·Reproductive Sciences·Leo DohertyHugh Taylor
Mar 12, 2010·Pharmaceuticals·Külliki SaarMargus Pooga
Jun 23, 2018·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Hong-Bin WangHua Zhi
May 20, 2020·Journal of Clinical Medicine·Michał CiebieraAyman Al-Hendy
Jun 28, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Binya LiuQin Yan

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