Gene therapy to enhance condylar growth using rAAV-VEGF

The Angle Orthodontist
Juan Dai, A Bakr M Rabie

Abstract

To test the hypothesis that the introduction of specific vascular growth inducting genes would favorably affect mandibular condylar growth in Sprague-Dawley (SD) rats over a limited experimental period. Therefore, the aim of this study is to examine the effect of gene therapy on condylar growth by means of a morphological assessment. Ninety 35-day-old female SD rats were randomly divided into three groups, which received any of the injections of recombinant adeno-associated virus mediated vascular endothelial growth factor (rAAV-VEGF), rAAV mediated enhanced green fluorescence protein (rAAV-eGFP), or phosphate-buffered saline (PBS) into both mandibular condyles. Each group of rats was sacrificed on the following experimental days: 7, 14, 21, 30, and 60. Left halves of the mandibles were isolated and digital pictures were obtained in a standardized manner. The length of condylar process (B-F) as well as mandibular length (A-F) significantly increased on day 30 and continued to increase until the end of the experiment. Moreover, the width of condyle (Q-R) had increased significantly from day 30 and lasted to day 60. Condylar length (C-D) was found to be significantly longer on day 60. Gene therapy with VEGF stimulates condylar gr...Continue Reading

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Citations

Apr 15, 2011·Gene Therapy·M Myakishev-RempelE M Schwarz
Jul 20, 2013·Tissue Engineering. Part B, Reviews·Nattharee ChanchareonsookJohn A Jansen
May 27, 2014·Injury·Ippokratis PountosPeter V Giannoudis
Jul 3, 2013·BioMed Research International·Tarek El-Bialy, Adel Alhadlaq
Dec 18, 2015·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·David S Carlson
Feb 5, 2015·Medical Hypotheses·M F N FeresT El-Bialy

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