Gene therapy with IgG-HY fusion proteins to reduce male-specific T-cell reactivity in vitro

Human Gene Therapy
Miranda P DierselhuisE Spierings

Abstract

Graft-versus-host disease is still a major complication in stem-cell transplantation. In HLA-identical stem-cell transplantation, mismatches in minor histocompatibility antigens contribute to the development of graft-versus-host disease. As treatment of graft-versus-host disease with immunosuppressive drugs potentially also reduces the graft-versus-leukemia responses, antigen-specific tolerance induction may be used to reduce graft-versus-host disease while leaving the graft-versus-leukemia responses intact. In mouse models, induction of antigen-specific tolerance using IgG-antigen-transduced B cells can prevent and ameliorate autoimmunity. In this study, the principle of the in vivo animal model has been applied to a human in vitro model wherein induction of tolerance against the HLA class II-restricted male-specific minor histocompatibility antigen RPS4Y1 has been analyzed. T cells precultured in the presence of B cells expressing the IgG-coupled HY antigen showed reduced responsiveness selectively against the minor HY antigen RPS4Y1 in different in vitro protocols. These first observations serve as a basis for further studying and understanding the tolerizing potential of IgG constructs with human T cells.

References

Apr 7, 1977·Nature·E GoulmyJ J van Rood
Aug 1, 1996·Human Gene Therapy·T M Kinsella, G P Nolan
Aug 17, 2000·Drug Safety : an International Journal of Medical Toxicology and Drug Experience·I Penn
Nov 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M El-AmineD W Scott
Sep 28, 2004·Transplantation·Brian J NankivellRichard D M Allen
Jan 8, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Martin S ZandNufatt Leong
Mar 16, 2005·Current Opinion in Immunology·Lothar Hambach, Els Goulmy
Sep 20, 2005·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Wei LiangDavid W Scott
Feb 14, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·S C JordanD Tyan
Aug 22, 2006·Autoimmunity Reviews·Daniel Brandt, M Eric Gershwin
Nov 23, 2006·Transplant Infectious Disease : an Official Journal of the Transplantation Society·J CarstensM Madsen
Dec 13, 2006·Transplant Immunology·Manuel Miras LópezPascual Parrilla Paricio
Jul 27, 2007·Nature Reviews. Immunology·Maria-Grazia Roncarolo, Manuela Battaglia
Aug 23, 2007·Transplant International : Official Journal of the European Society for Organ Transplantation·Cherry I KingsleyKathryn J Wood
Oct 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Aharon KesselElias Toubi
Nov 6, 2007·Current Gene Therapy·Jonathan SkupskyDavid W Scott
Jul 11, 2008·Immunological Reviews·Sarah E AllanMegan K Levings
Mar 14, 2009·Lancet·James L M FerraraErnst Holler
May 21, 2010·Molecular Therapy : the Journal of the American Society of Gene Therapy·Jonathan SkupskyDavid W Scott

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Citations

Sep 5, 2012·Gender Medicine·Steven Wagner
Apr 26, 2014·Haemophilia : the Official Journal of the World Federation of Hemophilia·D W Scott

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Methods Mentioned

BETA
PCR
FCS
FACS

Software Mentioned

CellQuest
GraphPad
GraphPad Prism

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