Gene transfection to spheroid culture system on micropatterned culture plate by polyplex nanomicelle: a novel platform of genetically-modified cell transplantation

Drug Delivery and Translational Research
Taisuke EndoKazunori Kataoka

Abstract

Three-dimensional (3D) cellular spheroids have attracted much attention as a transplantation procedure because the increased cell-to-cell interaction in spheroids enhances cell survival and its functions after the transplantation into the body. Furthermore, the potency of spheroidal cells may be further improved by introducing transgenes to augment cellular functions as well as enhance the paracrine effects by secreting key proteins involved in the essential cellular signaling cascades. In this study, we organized a new platform for genetically-modified cell transplantation by combining a microfabricated culture system for 3D spheroid formation with a newly developed non-viral transfection system, polyplex nanomicelle. After transfection of Gaussia luciferase using the nanomicelle, the prolonged luciferase expression was obtained for more than a month with continuous albumin secretion from the hepatocyte spheroids to the level comparable with control spheroids receiving no transfection. In contrast, by the transfection using FuGENE HD, a commercially available lipid-based reagent, the luciferase expression was obtained, yet the albumin secretion was significantly decreased with disintegration of the spheroid architecture. To as...Continue Reading

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Citations

Jan 21, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Frederico PittellaKazunori Kataoka

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