Gene transfer: how can the biological barriers be overcome?

The Journal of Membrane Biology
Jean-Michel EscoffreMarie-Pierre Rols

Abstract

Physical methods represent a promising approach for the safe delivery of therapeutic plasmid DNA in genetic and acquired human diseases. However, their development in clinics is limited by their low efficacy. At the cellular level, efficient gene transfer is dependent on several factors including extracellular matrix, plasmid DNA uptake and nucleocytoplasmic transport. We review the main barriers that plasmid DNA encounters from the extracellular environment toward the interior of the cell and the different strategies developed to overcome these biological barriers. Diffusional and metabolic fences of the extracellular matrix and the cytoplasm affect plasmid DNA uptake. These barriers reduce the number of intact plasmids that reach the nucleus. Nuclear uptake of plasmid DNA further requires either an increase of nuclear permeability or an active nuclear transport via the nuclear pore. A better understanding of the cellular and molecular bases of the physical gene-transfer process may provide strategies to overcome those obstacles that highly limit the efficiency and use of gene-delivery methods.

References

Sep 1, 1992·Analytical Biochemistry·P A FurthL Hennighausen
Mar 23, 1990·Science·J A WolffP L Felgner
Jan 17, 1991·Biochimica Et Biophysica Acta·A V TitomirovE Kistanova
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·W TaoM W Berns
Feb 1, 1986·Experimental Cell Research·S KurataY Ikawa
Dec 29, 1972·The Journal of Membrane Biology·E Neumann, K Rosenheck
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·T W DubenskyL P Villarreal
Apr 1, 1995·Hybridoma·P A FurthL Hennighausen
Apr 1, 1995·The Journal of Thoracic and Cardiovascular Surgery·A ArdehaliA J Lusis
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·M E DowtyJ A Wolff
May 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·L ChengN S Yang
Jul 8, 1996·FEBS Letters·R HellerC Nicolau
Jul 14, 1997·The Journal of Cell Biology·O SeksekA S Verkman
Oct 10, 1997·Human Gene Therapy·K A Choate, P A Khavari
Mar 6, 1998·Nature Biotechnology·M P RolsJ Teissie
Jul 4, 1998·Ultrasound in Medicine & Biology·W J GreenleafJ F Greenleaf
Sep 22, 1998·Nature Biotechnology·H Aihara, J Miyazaki
Apr 14, 1999·Proceedings of the National Academy of Sciences of the United States of America·L M MirD Scherman
Jul 31, 1999·Human Gene Therapy·J Glasspool-Malone, R W Malone
Oct 12, 1999·Gene Therapy·D SawamuraI Hashimoto
Jan 5, 2000·Proceedings of the National Academy of Sciences of the United States of America·T GotoY Ushio
Jan 15, 2000·The Journal of Biological Chemistry·G L LukacsA S Verkman
Apr 26, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·G WideraJ B Ulmer
May 16, 2000·The Journal of Gene Medicine·V BudkerJ A Wolff
Jun 30, 2000·Analytical Biochemistry·R CartierP A Furth
Aug 18, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M DupuisD M McDonald
Aug 19, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·J Glasspool-MaloneR W Malone
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·D J Stephens, R Pepperkok
Mar 29, 2001·Proceedings of the National Academy of Sciences of the United States of America·A PluenR K Jain
Mar 29, 2001·Experimental Hematology·E PayenD Scherman
Jan 11, 2002·Developmental Biology·T Saito, N Nakatsuji
Feb 28, 2002·Human Gene Therapy·Carmela MennuniNicola La Monica
May 15, 2002·Biochimica Et Biophysica Acta·Muriel GolzioMarie-Pierre Rols
May 29, 2002·The EMBO Journal·Katharina Ribbeck, Dirk Görlich
Jun 13, 2002·Molecular Biotechnology·Wolfgang WaltherPeter M Schlag

❮ Previous
Next ❯

Citations

Mar 29, 2014·Journal of the Royal Society, Interface·Xian ChenAlfred C H Yu
May 27, 2014·Therapeutic Delivery·Zhenzhen FanCheri X Deng
Apr 24, 2014·Critical Reviews in Microbiology·Ana Sofia DuarteAna Cristina Esteves
Feb 22, 2012·Expert Opinion on Biological Therapy·Saulius SatkauskasMindaugas S Venslauskas
Jun 1, 2014·Journal of Drug Targeting·Aya ZeghimiJean-Michel Escoffre
Aug 19, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Meihua SuiYouqing Shen
Sep 20, 2011·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Amir K VarkouhiWim E Hennink
May 24, 2011·International Journal of Pharmaceutics·Christelle RosazzaMarie-Pierre Rols
Jul 9, 2013·The Journal of Membrane Biology·Louise ChopinetDamijan Miklavčič
Aug 20, 2014·The Journal of Membrane Biology·L PasquetJ Teissie
Mar 20, 2015·The Journal of Membrane Biology·Moinecha MadiLaure Gibot
May 24, 2015·General and Comparative Endocrinology·María José MazónAna Gómez
Sep 10, 2014·Bioelectrochemistry·Bostjan MarkelcMaja Cemazar
Jun 19, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Z FanC X Deng
Sep 29, 2012·Journal of Controlled Release : Official Journal of the Controlled Release Society·Elisabeth BellardMuriel Golzio
Oct 12, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Tanja BlagusGregor Sersa
Aug 28, 2016·Journal of Controlled Release : Official Journal of the Controlled Release Society·M Rachèl ElzesJos M J Paulusse
Dec 3, 2016·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Yoav HadasLior Zangi
Jul 3, 2013·Journal of Laboratory Automation·J Mark MeachamAndrei G Fedorov
Apr 8, 2014·Biomedicines·Thomas Wirth, Seppo Ylä-Herttuala
Aug 7, 2013·Lab on a Chip·Tao Geng, Chang Lu
Oct 7, 2018·Bulletin of Mathematical Biology·Manon DevilleClair Poignard

❮ Previous
Next ❯

Related Concepts

Related Feeds

CREs: Gene & Cell Therapy

Gene and cell therapy advances have shown promising outcomes for several diseases. The role of cis-regulatory elements (CREs) is crucial in the design of gene therapy vectors. Here is the latest research on CREs in gene and cell therapy.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Muriel GolzioMarie-Pierre Rols
European Journal of Surgical Oncology : the Journal of the European Society of Surgical Oncology and the British Association of Surgical Oncology
G SersaM Snoj
© 2021 Meta ULC. All rights reserved