Gene Transfer into Pluripotent Stem Cells via Lentiviral Transduction

Methods in Molecular Biology
Ortwin NaujokMatthias Elsner

Abstract

Recombinant lentiviral vectors are powerful tools to stably manipulate human pluripotent stem cells. They can be used to deliver ectopic genes, shRNAs, miRNAs, or any possible genetic DNA sequence into diving and nondividing cells. Here we describe a general protocol for the production of self-inactivating lentiviral vector particles and their purification to high titers by either ultracentrifugation or ultrafiltration. Next we provide a basic procedure to transduce human pluripotent stem cells and propagate clonal cell lines.

Citations

Jan 16, 2019·In Vitro Cellular & Developmental Biology. Animal·Ji Hun LeeSeung Pyo Gong
Mar 2, 2019·Reports of Practical Oncology and Radiotherapy : Journal of Greatpoland Cancer Center in Poznań and Polish Society of Radiation Oncology·Patrycja CzerwińskaMaciej Wiznerowicz

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