PMID: 9527189Apr 4, 1998Paper

Gene transfer into the donor heart during cold preservation for heart transplantation

The Annals of Thoracic Surgery
S GojoS Kitamura

Abstract

Ex vivo gene transfer to heart grafts may hold promise as a means of changing alloreactivity or xenoreactivity after transplantation. However, it remains to be determined how effectively gene transfer can be accomplished within a short time in cold-stored grafts that are ready to be transplanted. We performed an experimental study using a replication-defective adenovirus (Adex1CALacZ) encoding the Escherichia coli beta-galactosidase (beta-gal) gene to perform gene transfer to heart grafts awaiting transplantation. Thirty hearts of Wistar rats were removed and their coronary arteries were perfused with University of Wisconsin solution containing 1 x 10(9), 1 x 10(10), or 1 x 10(11) plaque-forming units of the recombinant adenovirus at 4 degrees C for 60 minutes. As a control, other hearts were perfused with University of Wisconsin solution with an adenoviral vector that did not contain the beta-gal gene (Adex1w1) for the same period. After perfusion, the grafts were implanted in the necks of syngeneic adult rats. The grafts were removed each week after transplantation and their expression of beta-gal was assessed by 5-bromo-4-chloro-3-indoyl-beta-D-galactoside staining. Successful gene transfer and expression of the beta-gal gen...Continue Reading

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Citations

Nov 18, 2000·The Annals of Thoracic Surgery·K NishizakiS Taniguchi
Mar 10, 2001·The Annals of Thoracic Surgery·T SakaguchiH Matsuda
Feb 28, 2001·Journal of Cardiac Surgery·J V Conte, W A Baumgartner
Sep 15, 2010·Journal of Molecular and Cellular Cardiology·Michael G KatzCharles R Bridges
Apr 4, 2007·Transplant International : Official Journal of the European Society for Organ Transplantation·Johannes M SchirmerChristopher G A McGregor
Aug 13, 2011·The Journal of Gene Medicine·Nalinda B WasalaDongsheng Duan
Dec 22, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·Y WangJ S Bromberg
Sep 22, 1999·Gene Therapy·N GiannoukakisP Robbins
Jan 23, 2003·The Journal of Gene Medicine·Frank GriscelliThierry Ragot
May 8, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Alexander SasseJürgen Schrader

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