Gene transfer of inducible nitric oxide synthase complementary DNA regresses the fibrotic plaque in an animal model of Peyronie's disease

Biology of Reproduction
Hugo H DavilaNestor Gonzalez-Cadavid

Abstract

The goal of the present study was to investigate the antifibrotic role of inducible nitric oxide synthase (iNOS) in Peyronie's disease (PD) by determining whether a plasmid expressing iNOS (piNOS) injected into a PD-like plaque can induce regression of the plaque. A PD-like plaque was induced with fibrin in the penile tunica albuginea of mice and then injected with a luciferase-expressing plasmid (pLuc), either alone or with piNOS, following luciferase expression in vivo by bioluminescence imaging. Rats were treated with either piNOS, an empty control plasmid (pC), or saline. Other groups were treated with pC or piNOS, in the absence of fibrin. Tissue sections were stained for collagen, transforming growth factor (TGF) beta1, and plasminogen-activator inhibitor (PAI-1) as profibrotic factors; copper-zinc superoxide dismutase (CuZn SOD) as scavenger of reactive oxygen species (ROS); and nitrotyrosine to detect nitric oxide reaction with ROS. Quantitative image analysis was applied. Both iNOS and xanthine oxido-reductase (XOR; oxidative stress) were estimated by Western blot analysis. Luciferase reporter expression was restricted to the penis, peaked at 3 days after injection, but continued for at least 3 wk. In rats receiving pi...Continue Reading

References

Jan 1, 1997·The Journal of Urology·C J DevineS M Schlossberg
Jan 1, 1997·The Journal of Urology·K D Somers, D M Dawson
Nov 20, 1997·The Journal of Clinical Investigation·C Nathan
Nov 14, 2000·Circulation Research·H Cai, D G Harrison
Nov 25, 2000·Laboratory Investigation; a Journal of Technical Methods and Pathology·D HochbergD Felsen
May 16, 2002·Nitric Oxide : Biology and Chemistry·M G FerriniN F Gonzalez-Cadavid
Jun 25, 2002·Microcirculation : the Official Journal of the Microcirculatory Society, Inc·Avedis Meneshian, Gregory B Bulkley
Nov 26, 2002·Nitric Oxide : Biology and Chemistry·Dolores VernetNestor F Gonzalez-Cadavid
Dec 31, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Trinity J BivalacquaHunter C Champion
Mar 12, 2003·The Journal of Urology·Shahram S GholamiTom F Lue
Mar 26, 2003·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·David T EfronAdrian Barbul
Nov 1, 2003·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Josef E Fischer
Mar 5, 2004·Nitric Oxide : Biology and Chemistry·Eliane G A ValenteNestor F Gonzalez-Cadavid

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Citations

Nov 16, 2004·Current Urology Reports·Mark JalkutJacob Rajfer
Feb 14, 2006·Nature Clinical Practice. Urology·Nestor F Gonzalez-Cadavid, Jacob Rajfer
Aug 4, 2007·International Journal of Impotence Research·A RambhatlaJ Rajfer
Oct 28, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Jiaming WenYang Xia
Sep 29, 2006·Journal of Neuroinflammation·Carol A ColtonMichael P Vitek
Jan 27, 2012·The Tohoku Journal of Experimental Medicine·Yong ZhangZhaodian Chen
Aug 5, 2006·Asian Journal of Andrology·David H W LauFaiz H Mumtaz
Sep 9, 2008·Expert Opinion on Biological Therapy·Serap GurWayne J G Hellstrom
Dec 14, 2007·Expert Opinion on Pharmacotherapy·Ahmed I El-Sakka, Aksam A Yassin
Feb 16, 2006·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Hugo E BeurdenAnne M Kuijpers-Jagtman
Apr 11, 2007·The Journal of Sexual Medicine·Trustin DomesGerald Brock
Jun 19, 2008·The Journal of Sexual Medicine·Neal MehtaMahadevan Rajasekaran
May 13, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·Hanzhong LiuGary A Visner
Jul 13, 2006·BJU International·Istvan KovaneczNestor F Gonzalez-Cadavid
Apr 16, 2011·Expert Opinion on Therapeutic Targets·Ibrahim A Abdel-Hamid, Tarek Anis
Dec 14, 2011·Medical Hypotheses·Serap GurWayne J G Hellstrom
Mar 26, 2013·The Journal of Urology·Tao WangLong-Cheng Li
Mar 10, 2010·Nature Reviews. Urology·Nestor F Gonzalez-Cadavid, Jacob Rajfer
Mar 1, 2011·Arab Journal of Urology·Ahmed I El-Sakka
Oct 17, 2019·International Urology and Nephrology·Ahmed Mohamed Hassanin, Ahmed Zain Abdel-Hamid
Feb 11, 2020·Clinical and Experimental Reproductive Medicine·Apichakan SampannangSitthichai Iamsaard
Jan 9, 2017·The World Journal of Men's Health·Hyong Woo MoonSae Woong Kim
Apr 22, 2008·The Journal of Sexual Medicine·Liliana P CantiniNestor F Gonzalez-Cadavid
Jan 14, 2009·The Journal of Sexual Medicine·Nestor F Gonzalez-Cadavid, Jacob Rajfer

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Methods Mentioned

BETA
bioluminescence

Software Mentioned

Living Image
ImagePro
GraphPad Prism

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