Gene transfer to the rodent placenta in situ. A new strategy for delivering gene products to the fetus

The Journal of Clinical Investigation
M C SenutF H Gage

Abstract

The delivery of biologically active factors to the developing mammalian embryo by in utero gene transfer has generated considerable interest but limited success. The chorioallantoic placenta is a potential alternative target for providing therapeutic transgenes to the fetus during gestation. We demonstrate that somatic gene transfer to the midgestation rat placenta may be efficiently accomplished in situ through the implantation of a variety of genetically modified cells with different antigenic and growth properties. Ex vivo-modified cells survived and retained transgene expression until term. Proteins secreted from the transplanted cells were detectable within the fetal trunk blood. These studies suggest that gene transfer to the placenta may be a useful tool for answering questions of both embryonic and placental development and providing therapeutic proteins during gestation for amelioration of diseases with onset during embryonic life.

References

Jul 1, 1977·The Journal of General Virology·F L GrahamR Nairn
Jan 1, 1987·Developmental Biology·M PoplikerC G Webb
Jun 1, 1995·The Journal of Clinical Investigation·P B McCrayM J Welsh
Aug 1, 1995·Human Gene Therapy·M C VincentH S Iwamoto
Nov 1, 1995·Nature Medicine·H S Sekhon, J E Larson
Sep 1, 1995·Nature Medicine·C CoutelleU Froster
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·M W HeartleinR F Selden
Jun 24, 1994·Science·J J LetterioA B Roberts
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·M HoshimaruF H Gage
Jul 1, 1996·Placenta·M J SoaresK E Orwig

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Citations

Mar 10, 2001·Mental Retardation and Developmental Disabilities Research Reviews·X YeM L Batshaw
May 18, 1999·Molecular Medicine Today·M C Senut, F H Gage
Jul 2, 2008·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yuka Okada
Nov 11, 1999·Proceedings of the National Academy of Sciences of the United States of America·G S LipshutzK M Gaensler
Aug 31, 2007·Expert Opinion on Biological Therapy·Anna KanervaAkseli Hemminki
Jan 25, 2011·Placenta·S J RenaudM J Soares
May 12, 2009·Advanced Drug Delivery Reviews·Anna M WagnerDaniel Surbek
Jun 10, 2008·Transfusion and Apheresis Science : Official Journal of the World Apheresis Association : Official Journal of the European Society for Haemapheresis·Rukset Attar, Erkut Attar
Apr 19, 2008·Seminars in Fetal & Neonatal Medicine·Daniel SurbekAnna Wagner
May 17, 2002·American Journal of Obstetrics and Gynecology·Annaleena HeikkiläSeppo Ylä-Herttuala
May 7, 2002·Molecular Therapy : the Journal of the American Society of Gene Therapy·Masayuki EndohYasufumi Kaneda
May 3, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Mari RakiAkseli Hemminki
Mar 29, 2001·Molecular Therapy : the Journal of the American Society of Gene Therapy·G S LipshutzK M Gaensler
Mar 8, 2019·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Jean-Sebastien WattezJianhua Shao
Nov 30, 2019·International Journal of Molecular Sciences·Shingo NakamuraMasahiro Sato
Nov 16, 2001·Current Rheumatology Reports·J F Beary
Feb 5, 2002·European Archives of Psychiatry and Clinical Neuroscience·J Bobes
Oct 16, 1999·Science·E D Zanjani, W F Anderson

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