Gene-Wide Analysis of Aquaporin Gene Family in Malus domestica and Heterologous Expression of the Gene MpPIP2;1 Confers Drought and Salinity Tolerance in Arabidposis thaliana

International Journal of Molecular Sciences
Haili LiuJingying Liu

Abstract

The aquaporins (AQPs) are a family of integral membrane proteins involved in the transcellular membrane transport of water and other small molecules. A scan of the apple (Malus domestica) genome revealed the presence of 42 genes encoding putative AQPs. Based on a phylogenetic analysis of the deduced peptide sequences of the AQPs generated by Arabidopsis thaliana, poplar (Populus trichocarpa), and rubber (Hevea brasiliensis), the apple AQPs were each assigned membership of the five established AQP subfamilies, namely the PIPs (eleven members), the TIPs (thirteen members), the NIPs (eleven members), the SIPs (five members), and the XIPs (two members). The apple AQPs included asparagine-proline-alanine (NPA) motifs, an aromatic/arginine (ar/R) selectivity filter, and the Froger's positions. The heterologous expression of MpPIP2;1 in A. thaliana was shown to enhance the level of tolerance exhibited against both drought and salinity.

References

Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Feb 5, 2000·Journal of Molecular Biology·J B Heymann, A Engel
Oct 7, 2004·Trends in Plant Science·Ron MittlerFrank Van Breusegem
May 18, 2005·Journal of Experimental Botany·Daniel A Eisenbarth, Alfons R Weig
Oct 3, 2006·The Plant Journal : for Cell and Molecular Biology·Jaume FlexasRalf Kaldenhoff
May 23, 2007·Nucleic Acids Research·Paul HortonKenta Nakai
Apr 24, 2008·BMC Plant Biology·Jonas A H Danielson, Urban Johanson
Dec 23, 2008·Plant & Cell Physiology·Tadashi MatsumotoYoshichika Kitagawa
Sep 28, 2010·Plant Physiology and Biochemistry : PPB·Sarvajeet Singh Gill, Narendra Tuteja
Feb 11, 2011·Plant Molecular Biology·Runyararo Memory Hove, Mrinal Bhave
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Dec 21, 2013·Biochimica Et Biophysica Acta·Federico AbascalRafael Zardoya
Jul 10, 2014·BMC Plant Biology·Carole Leavel BassettRobert E Farrell
Dec 17, 2014·Bioinformatics·Bo HuGe Gao
Feb 24, 2015·Biochemical and Biophysical Research Communications·Jun LiXuemin Wang
Jul 15, 2015·The Plant Cell·Alexandre GrondinChristophe Maurel
Aug 11, 2015·Plant Science : an International Journal of Experimental Plant Biology·Francisco J Molina-HidalgoRosario Blanco-Portales
Sep 8, 2015·Current Opinion in Structural Biology·Stefan Kreida, Susanna Törnroth-Horsefield
Oct 4, 2017·Plant Science : an International Journal of Experimental Plant Biology·Ana Romina FoxFrançois Chaumont
Aug 1, 2018·International Journal of Molecular Sciences·Wei LiXian-Guo Cheng
Aug 31, 2018·Cells·Avat Shekoofa, Thomas R Sinclair

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
transgenics
transgenic
MDA
Assay

Software Mentioned

SPSS
TMHMM
ExPASY
BLASTN
Beacon Designer
GSDS
WoLF PSOR
MEGA6
ClustalX

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.