PMID: 20657155Jul 27, 2010Paper

General features of certain RNA populations from gametes and cumulus cells

The Journal of Reproduction and Development
Rebecca R PaytonJ Lannett Edwards

Abstract

Results described herein provide insight regarding certain features of gamete RNA and how they compare to cumulus cell RNA. In particular, 28S/18S rRNA ratio and size distribution of RNA molecules differed in total RNA from oocytes versus surrounding cumulus cells. Specifically, oocyte total RNA had a lower rRNA ratio and an increased abundance of smaller RNA sizes compared to RNA from surrounding cumulus. Extensive efforts demonstrated that observed differences were repeatable whether oocyte maturation occurred in vitro or in vivo, and were similar between the nuclear stages examined. Features of oocyte RNA were conserved across six mammalian species, yet differed from surrounding cumulus. Profiles of sperm RNA were also examined but had no discernible ribosomal RNA peaks and were conserved across four mammalian species. Because the oocyte and spermatozoon are highly specialized cells representing unique molecular entities required for proper embryo development, dissimilarities described herein likely represent real gamete versus cumulus RNA differences.

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Citations

Apr 12, 2011·The Journal of Reproduction and Development·Rebecca R PaytonJ Lannett Edwards
Sep 22, 2011·Zygote : the Biology of Gametes and Early Embryos·P M M LeonT Collares
Jan 9, 2013·Mechanisms of Development·Valérie S Langlois, Christopher J Martyniuk
Aug 15, 2020·Scientific Reports·Lais B LatorracaFabíola F Paula-Lopes

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