PMID: 6970289Jan 1, 1980Paper

General properties of antimony microelectrode in comparison with glass microelectrode for pH measurement

The Japanese Journal of Physiology
M FujimotoN Satake

Abstract

Two kinds of pH-sensitive microelectrodes were constructed: 1) glass microelectrode with a pH-sensitive tip of 50--100 micrometers in length, and 2) antimony (Sb) microelectrode with a tip of 1--5 micrometers in outside diameter. Comparisons of the two were made on the change in the electromotive force (EMF) in response to various pHs and biological applicability. The pH sensitivity (slope constant) at 20 degrees C of the glass and Sb-microelectrodes averaged 51.7 and 51.9 (-mV/pH), respectively. Over the pH range of 2 to 9, both electrodes responded linearly to the change of pH. The pH-dependent EMF of the Sb-microelectrode was influenced by phosphate buffers, but not by Tris-HCl buffers. Through the glass microelectrode showed a linear response to all the buffers tested, its slope constant tended to be limited by the surface area of the pH-sensitive tip. The stability of the EMFs was well within 1 mV for 12 hr with the glass microelectrode but more than 10 mV with the Sb-microelectrode. One the other hand, the 95% response time was about 1 min with the glass microelectrode, whereas it was less than a few seconds with the Sb-microelectrode. Blood pH was measured with the two electrodes and reasonable values were obtained with ...Continue Reading

Citations

Sep 1, 1988·Medical & Biological Engineering & Computing·M F Kiani, R W Schubert
Nov 2, 2002·The Journal of Physiology·Heidi K BaumgartnerMarshall H Montrose
Jan 1, 1989·Critical Reviews in Analytical Chemistry·StanisŁAw GłabFolke Ingman

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