PMID: 9532267Apr 9, 1998Paper

Generalized transduction for genetic linkage analysis and transfer of transposon insertions in different Staphylococcus epidermidis strains

Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology
M NedelmannDietrich Mack

Abstract

Staphylococcus epidermidis phage 48 was used to efficiently transduce plasmid pTV1ts and a chromosomal Tn917 insertion M27 from S. epidermidis 13-1 to biofilm-producing clinical S. epidermidis isolates 1457, 9142, and 8400. The Tn917 insertion leading to the biofilm-negative phenotype of transposon mutant M10 was sequentially transduced to biofilm-producing S. epidermidis 1457 using S. epidermidis phage 48 and then, using the resulting biofilm-negative transductant 1457-M10 as a donor, into several unrelated biofilm-producing clinical S. epidermidis isolates using S. epidermidis phage 71. All resultant transductants displayed a completely biofilm-negative phenotype. In addition, S. epidermidis phage 71 was adapted to S. epidermidis 1457 and 8400, which allowed generalized transduction of transposon insertions in these wild-type strains. As Tn917 predominantly transposed into endogenous plasmids of all three strains used, an efficient system for chromosomal transposon mutagenesis was established by curing of S. epidermidis 1457 of a single endogenous plasmid p1457 by sodium dodecylsulfate treatment. After transduction of the resulting derivative, S. epidermidis 1457c with pTV1ts, insertion of transposon Tn917 to different sites ...Continue Reading

References

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Nov 1, 1971·Journal of Medical Microbiology·J VerhoefC P van Boven
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Citations

Feb 5, 2000·The Journal of Hospital Infection·D Mack
Oct 4, 2011·Microbiology·Marat R SadykovGreg A Somerville
Jan 27, 2015·Applied and Environmental Microbiology·Volker WinstelHolger Rohde
May 4, 2011·Microbial Biotechnology·Maite MuniesaJuan Jofre
Jun 3, 2017·Genome Announcements·Madeline R GalacPaul D Fey

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