Abstract
Staphylococcus epidermidis phage 48 was used to efficiently transduce plasmid pTV1ts and a chromosomal Tn917 insertion M27 from S. epidermidis 13-1 to biofilm-producing clinical S. epidermidis isolates 1457, 9142, and 8400. The Tn917 insertion leading to the biofilm-negative phenotype of transposon mutant M10 was sequentially transduced to biofilm-producing S. epidermidis 1457 using S. epidermidis phage 48 and then, using the resulting biofilm-negative transductant 1457-M10 as a donor, into several unrelated biofilm-producing clinical S. epidermidis isolates using S. epidermidis phage 71. All resultant transductants displayed a completely biofilm-negative phenotype. In addition, S. epidermidis phage 71 was adapted to S. epidermidis 1457 and 8400, which allowed generalized transduction of transposon insertions in these wild-type strains. As Tn917 predominantly transposed into endogenous plasmids of all three strains used, an efficient system for chromosomal transposon mutagenesis was established by curing of S. epidermidis 1457 of a single endogenous plasmid p1457 by sodium dodecylsulfate treatment. After transduction of the resulting derivative, S. epidermidis 1457c with pTV1ts, insertion of transposon Tn917 to different sites ...Continue Reading
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Citations
Dec 26, 2001·Antimicrobial Agents and Chemotherapy·Dietrich MackJohannes K-M Knobloch
Feb 25, 2005·Antimicrobial Agents and Chemotherapy·Johannes K-M KnoblochDietrich Mack
Oct 9, 2003·Applied and Environmental Microbiology·Johannes K-M KnoblochDietrich Mack
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Sep 11, 2012·Molecular Microbiology·Martin ChristnerHolger Rohde
Dec 1, 2009·Molecular Microbiology·Martin ChristnerHolger Rohde
Feb 5, 2000·The Journal of Hospital Infection·D Mack
Jul 24, 2014·Journal of Medical Microbiology·Filipe CercaManuel Vilanova
Oct 4, 2011·Microbiology·Marat R SadykovGreg A Somerville
Oct 22, 2014·Infection and Immunity·Carolyn R SchaefferPaul D Fey
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