DOI: 10.1101/489641Dec 9, 2018Paper

Generating closed bacterial genomes from long-read nanopore sequencing of microbiomes

BioRxiv : the Preprint Server for Biology
Eli L Moss, Ami S Bhatt


We present the first method for efficient recovery of complete, closed genomes directly from microbiomes using nanopore long-read sequencing and assembly. We apply our approach to three healthy human gut communities and compare results to short read and read cloud approaches. We obtain nine finished genomes including the first reported closed genome of Prevotella copri, an organism with highly repetitive genome structure prevalent in non-western human gut microbiomes.

Related Concepts

Molecular Assembly/Self Assembly
Prevotella copri
Nucleic Acid Sequencing

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

BioRxiv : the Preprint Server for Biology
Alex BisharaAmi S Bhatt
Nature Biotechnology
David DeamerDaniel Branton
Nature Biotechnology
John J Kasianowicz, Sergey M Bezrukov
© 2021 Meta ULC. All rights reserved