Abstract
Lymphatic vessels, by channeling fluid and leukocytes from the periphery into lymph nodes, play a central role in the development of the immune response. Despite their importance in homeostasis and disease, the difficulties in enriching and culturing lymphatic endothelial cells limit studies of their biology. Here, we report the isolation, stabilization, and characterization of a mouse lymphatic endothelial cell line (MELC) and the generated clones thereof. Cells were isolated from benign lymphangiomas induced by intraperitoneal injections of incomplete Freund's adjuvant. The MELC line expressed molecules typical of lymphatic endothelium, including VEGFR3/Flt-4, podoplanin, Prox-1, and D6, but not LYVE-1. It also expressed CD34, ICAM-1, VCAM, and JAM-A, but not CD31, VE-cadherin, E-selectin, or CX3CL1/fractalkine (both TNFalpha-induced), at variance with vascular endothelial cells tested in parallel. The inflammatory cytokines TNFalpha and IL-4 regulated production of selected adhesion molecules (VCAM), cytokines (IL-6), and chemokines (CCL2/JE). Whole genome transcriptional profiling identified a set of 150 known genes differentially expressed in MELC versus vascular endothelial cells. Thus, the MELC line may represent an inva...Continue Reading
References
May 1, 1992·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A MantovaniE Dejana
Jan 15, 1988·Cell·R L WilliamsE F Wagner
Sep 15, 1994·Blood·M SironiF Colotta
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·A KaipainenK Alitalo
Jul 19, 1994·Proceedings of the National Academy of Sciences of the United States of America·C GarlandaA Vecchi
Aug 1, 1997·Arteriosclerosis, Thrombosis, and Vascular Biology·Q G DongA Vecchi
Jul 14, 1998·The Journal of Cell Biology·I Martìn-PaduraE Dejana
Jul 31, 1998·British Journal of Cancer·J P van NettenR P Finegan
Sep 2, 1998·Cell and Tissue Research·M SchmelzR Moll
Feb 2, 1999·Experimental Cell Research·S MancardiO R Burrone
Feb 2, 1999·The Journal of Experimental Medicine·M D GunnH Nakano
Feb 23, 1999·The American Journal of Pathology·S Breiteneder-GeleffD Kerjaschki
Sep 28, 1999·Cell·J T Wigle, G Oliver
Aug 10, 2000·Histopathology·E SinzelleP Bruneval
Oct 12, 2000·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·T A PartanenK Alitalo
Feb 24, 2001·European Radiology·C CarreiraJ Pinto
Mar 10, 2001·The American Journal of Pathology·R J NibbsA Rot
May 30, 2001·Trends in Immunology·D G JacksonS Banerji
May 30, 2001·Trends in Immunology·A MantovaniP Allavena
Sep 5, 2001·The EMBO Journal·T MäkinenK Alitalo
Sep 19, 2001·The Journal of Experimental Medicine·E KriehuberD Maurer
Oct 12, 2001·Microscopy Research and Technique·M H WitteC L Witte
Dec 18, 2001·The Journal of Pathology·A N WitmerR O Schlingemann
Jan 16, 2002·Anatomy and Embryology·M Rodriguez-NiedenführJ Wilting
Jun 28, 2002·Cancer Cell·Kari Alitalo, Peter Carmeliet
Jul 20, 2002·Blood·Monica CoradaElisabetta Dejana
Aug 29, 2002·The EMBO Journal·Tatiana V PetrovaKari Alitalo
Nov 26, 2002·Proceedings of the National Academy of Sciences of the United States of America·Simona PodgrabinskaMihaela Skobe
Jan 30, 2003·Trends in Cardiovascular Medicine·David G Jackson
Apr 2, 2003·Immunology·Sabrina MancardiAldo Dobrina
Jun 24, 2003·The American Journal of Pathology·Pedram HamrahM Reza Dana
Aug 20, 2003·Nature Immunology·David WaldXiaoxia Li
Mar 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Cecilia GarlandaAlberto Mantovani
Mar 9, 2004·Nature Immunology·Elizabeth K BrintFoo Y Liew
Jun 29, 2004·Nature Genetics·Hsei-Wei WangChris Boshoff
Citations
May 28, 2013·Angiogenesis·Imke AlbrechtGerhard Christofori
Nov 27, 2007·Nature Immunology·Levi G LedgerwoodJonathan S Bromberg
Sep 12, 2007·The Journal of Experimental Medicine·Peter BalukDonald M McDonald
Mar 11, 2009·The Journal of Experimental Medicine·Luigi MaddalunoUgo Cavallaro
Oct 13, 2006·Lymphatic Research and Biology·Brandt WhitehurstSophia Ran
Oct 16, 2007·Lymphatic Research and Biology·Amarendra PeguTodd A Reinhart
May 19, 2007·Lymphatic Research and Biology·F Blei
Jul 1, 2011·Journal of Biomedicine & Biotechnology·Federica MoalliCecilia Garlanda
Jul 14, 2012·PloS One·Jan KazenwadelNatasha L Harvey
Sep 1, 2008·Expert Review of Clinical Immunology·Lianping Xing, Rui-Cheng Ji
Aug 16, 2011·Experimental Cell Research·Sanja CosoElizabeth D Williams
Mar 23, 2011·Journal of Comparative Pathology·Y SunY Zhao
Oct 27, 2015·Experimental Eye Research·Xianling TangPing Liu
Feb 24, 2016·Leukemia·L ZanottiA Viola
Sep 8, 2007·Microvascular Research·Takeshi UekiYoshihiko Sawa
Jun 4, 2008·Annals of the New York Academy of Sciences·Peter Baluk, Donald M McDonald
Sep 20, 2007·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Philipp KastenJörg Wilting
Nov 11, 2011·Cell Biology International·Peiliang Wang, Yongbo Cheng
Mar 11, 2011·Tissue Antigens·A InforzatoC Garlanda
Feb 27, 2010·Proteomics·Vicky P K H NguyenDaniel J Dumont
Mar 10, 2015·Molecular Cancer·Elena Tutunea-FatanPeeyush K Lala
Feb 28, 2016·Journal of Hepatology·Barbara BottazziAlberto Mantovani
May 6, 2014·Immunology Letters·Kenji DaigoBarbara Bottazzi
Jun 12, 2013·Seminars in Immunology·Antonio InforzatoAlberto Mantovani
Feb 11, 2014·The American Journal of Pathology·Alessandra DecioDorina Belotti
Oct 8, 2011·Blood·Kit Ming LeeGerard J Graham
Dec 30, 2014·Veterinary Immunology and Immunopathology·Stella J BerendamTodd A Reinhart
Aug 25, 2016·Journal of Ethnopharmacology·Jinlong LiQianqian Liang
Sep 23, 2016·Frontiers in Immunology·Kenji DaigoTakao Hamakubo
Jul 12, 2007·Pharmacology·Jun WangJian-Fei Gao
Jan 5, 2017·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Wensheng WangLianping Xing
Aug 26, 2018·Arthritis & Rheumatology·Wensheng WangLianping Xing
Jan 28, 2018·Scientific Reports·Maurice SwinkelsSimon J Clark
Sep 4, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Françoise Bruyère, Agnès Noël
Oct 14, 2017·Immunological Reviews·Marco ErreniPatrizia Rovere-Querini
Jun 18, 2020·Frontiers in Physiology·Feng ZhangAnne Eichmann
Jan 1, 2015·Arthritis Research & Therapy·Qianqian LiangLianping Xing
Jan 29, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Safiye Gonzalvo-FeoSilvano Sozzani
Feb 20, 2021·Arthritis Research & Therapy·Qianqian LiangLianping Xing
Jul 21, 2021·Nature Communications·Dali ZhangYangli Xie