Generation and characterization of a polyclonal antipeptide antibody to human glycodelin

Fertility and Sterility
A S PoddarS Parthasarathy

Abstract

To develop and characterize an antiglycodelin antibody using a 15-amino acid synthetic peptide as antigen, derived from the sequence of human glycodelin. We have developed a chicken antiglycodelin-derived peptide antibody and have characterized the antibody with the use of endometrial and ovarian cell lines. The antibody was also tested for its ability to detect glycodelin by ELISA assay, immunocytochemistry, and by Western blot. Various cell lines, cell culture medium, and amniotic fluid were used in the experiments. Amniotic fluid was collected from pregnant patients in their first trimester of pregnancy. No intervention. Detection of glycodelin. The cell lines RL95-2 (human endometrial carcinoma cells), OVCAR-3 (human ovarian adenocarcinoma cells), HeLa (human cervical epitheloid carcinoma cells), and EM42-D (human endometrial epithelial cells) reacted with the antibody, indicating the presence of glycodelin. A specific 45-kd protein representing glycodelin was detected by Western blot in the amniotic fluid. Antipeptide antibodies can be successfully used to detect and quantify the presence of glycodelin in cells and fluids.

References

Jan 1, 1992·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·L Riittinen
Dec 1, 1991·American Journal of Reproductive Immunology : AJRI·N OkamotoT Mori
May 1, 1986·Endocrinology·M JulkunenM Seppälä
Jan 1, 1985·Annals of the New York Academy of Sciences·M SeppäläT Wahlström
Oct 1, 1984·British Journal of Obstetrics and Gynaecology·E M RutanenM Seppälä
Feb 1, 1996·Human Reproduction·K Gemzell-DanielssonM Seppälä

❮ Previous
Next ❯

Citations

May 21, 2005·Archives of Gynecology and Obstetrics·Manoj Kumar PandeySuraksha Agrawal
Oct 15, 2008·Histochemistry and Cell Biology·Udo JeschkeKlaus Friese
Feb 11, 2005·Journal of Molecular Histology·Christiane Kunert-KeilJürgen Giebel
Jun 16, 2007·Irish Journal of Medical Science·E E Lang, G Venkatraman
Apr 28, 2000·Fertility and Sterility·S SawatsriN Sidell
Feb 5, 2002·Cancer Letters·Sumathi RamachandranSampath Parthasarathy
Mar 26, 2003·Free Radical Biology & Medicine·Sumathi RamachandranSampath Parthasarathy
Jul 5, 2001·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·I R HorowitzS Ramachandran
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·M SongS Parthasarathy
Nov 30, 2006·American Journal of Obstetrics and Gynecology·John K ParkAna A Murphy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society
I R HorowitzSumathi Ramachandran
Proceedings of the National Academy of Sciences of the United States of America
M SongS Parthasarathy
© 2022 Meta ULC. All rights reserved