Generation and characterization of alpha-chymase-Cre transgenic mice

Genesis : the Journal of Genetics and Development
Werner MüschThomas Hehlgans


The Cre-loxP technology allows the introduction of somatic gene alterations in a tissue and/or cell type specific manner. The development of transgenes that target Cre expression to specific cell types is a critical component in this system. Here, we describe the generation and characterization of transgenic mouse lines expressing Cre recombinase under the control of the baboon alpha-chymase promoter, designated Chm:Cre, in order to direct Cre expression specifically to mouse mast cells. Chm:Cre expression was detected in mast cells in lung and colon tissue. Cre-mediated recombination in these mice identified a population of mature tissue resident mast cells using ROSA26R reporter mice. No Cre-expression and Cre-mediated recombination was induced in in vitro generated bone marrow derived mast cells or mast cells isolated from the peritoneal cavity indicating that Cre-expression under the control of the alpha-chymase promoter is solely activated in tissue resident mast cells. These Chm:Cre transgenic mice represent a useful tool to specifically inactivate genes of interest in mast cells of these tissues.


Apr 25, 2012·Cancer Immunology, Immunotherapy : CII·Dyana K Dalton, Randolph J Noelle
Jan 19, 2013·Cell Death & Disease·Katja WoidackiAna Zenclussen
Oct 22, 2008·Nature Immunology·Janet Kalesnikoff, Stephen J Galli
Apr 6, 2013·Nature Reviews. Immunology·David Voehringer
Jul 8, 2009·Current Opinion in Organ Transplantation·Victor C de VriesRandolph J Noelle
Feb 19, 2014·Reproduction : the Official Journal of the Society for the Study of Fertility·Franziska SchmerseAna Zenclussen
Feb 4, 2014·Pharmacology & Therapeutics·Laurent L Reber, Nelly Frossard
May 27, 2014·Journal of Immunological Methods·Clare L AbramClifford A Lowell
Nov 12, 2010·Cellular and Molecular Life Sciences : CMLS·Anders Lundequist, Gunnar Pejler
Nov 7, 2012·Trends in Immunology·Laurent L ReberStephen J Galli
Dec 17, 2008·Clinical and Experimental Immunology·A WasiukRandolph J Noelle
Sep 25, 2014·Allergy·Atsushi Otsuka, Kenji Kabashima
Oct 5, 2010·Current Opinion in Immunology·Victor C de Vries, Randolph J Noelle
Feb 12, 2015·Mucosal Immunology·Laurent L ReberStephen J Galli
Jul 31, 2012·Immunity·Hans-Reimer Rodewald, Thorsten B Feyerabend
Dec 15, 2010·Biochimica Et Biophysica Acta·J HeijmansG R van den Brink
Mar 18, 2010·Blood·Gunnar PejlerSara Wernersson
May 14, 2016·Seminars in Immunopathology·Atsushi OtsukaKenji Kabashima
Jun 29, 2017·Immunological Reviews·Atsushi OtsukaKenji Kabashima
Jan 24, 2019·Laboratory animal research·Hyeonhui KimSungsoon Fang
Nov 5, 2019·Frontiers in Immunology·Ying LuoFrank Siebenhaar


Jan 26, 1996·Science·U M ChandrasekharanA Husain
Jan 31, 1997·The Journal of Biological Chemistry·Y LiaoA Husain
Mar 15, 1997·Nucleic Acids Research·R C RickertK Rajewsky
Jun 24, 2003·Genesis : the Journal of Genetics and Development·Véronique DelmasLionel Larue
Jun 10, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Peter StopferThomas Hehlgans

Related Concepts

Cre recombinase, Enterobacteria phage P1
Taenia Coli
Mast Cell
RNA, Untranslated
Gt(ROSA)26Sor non-coding RNA, mouse
Bone Marrow
Recombination, Genetic

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