PMID: 9420858May 1, 1995Paper

Generation and characterization of antibodies against synthetic peptides of porcine zona pellucida ZP3 alpha

Journal of the Society for Gynecologic Investigation
L L HallK S Moghissi

Abstract

Use of synthetic zona peptides as target immunogens is a promising approach to an anti-fertility vaccine. To elucidate contraceptive epitopes, we raised polyclonal antibodies against synthetic peptides of pig ZP3 alpha and evaluated for immunogenicity, ability to inhibit in vitro boar sperm-pig zona attachment, and cross-reactivity with human zonae. Five synthetic peptides were chosen based on hydropathicity analysis of ZP3 alpha, synthesized and coupled to keyhold limpet haemocyanin (KLH). Pairs of male rabbits were immunized with each peptide-KLH conjugate using a multi-site intradermal injection protocol. Resulting antisera were evaluated for immunoreactivity with cognate peptide and ZP3 alpha/EBGD (purified endo-beta-galactosidase-digested ZP3 alpha), contraceptive potential using an in vitro pig gamete bioassay, and cross-reactivity with human zonae using indirect immunofluorescence. All anti-peptide-KLH sera demonstrated immunoreactivity with cognate peptide. Anti-peptide sera, except for anti-alpha-3-KLH serum, were less immunoreactive with ZP3 alpha/ EBGD. Antisera directed against alpha-1-KLH, alpha-2-KLH, and alpha-3-KLH demonstrated significant (P < .00001) inhibition of boar sperm-pig zona attachment. Only anti-alph...Continue Reading

Citations

Nov 2, 1999·Micron : the International Research and Review Journal for Microscopy·J R Harris, J Markl
Feb 22, 2000·Journal of Drug Targeting·R I Mahato
Oct 13, 2000·American Journal of Respiratory and Critical Care Medicine·A J GeorgeD F Larkin

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