Generation and characterization of novel monoclonal antibodies against human aurora-A.

Hybridoma
Liming WuJunjie Chen

Abstract

The mitotic kinase Aurora-A is essential for mitotic progression, including centrosome maturation, mitotic spindle formation, and faithful segregation of chromosomes to daughter cells. Several lines of evidences also suggest that the mammalian aurora kinase family proteins play a role in tumorigenesis. We have previously shown that human Aurora-A was ubiquitinated and negatively regulated by an early mitotic checkpoint protein, Chfr (checkpoint protein with FHA and RING domain). Here, we established several mouse anti-Aurora-A monoclonal antibodies (MAb). GST-tagged human Aurora-A was expressed in BL21 and used as an antigen to immunize mice. Three different hybridomas were obtained and antibodies produced by these hybridomas were analyzed. The results reveal that these antibodies specifically recognize endogenous Aurora-A in both immunoblotting and immunofluroscence experiments. They are useful tools for further analysis of human Aurora-A.

References

Oct 26, 2000·Japanese Journal of Cancer Research : Gann·T TakahashiS Saji
Apr 6, 2001·International Journal of Cancer. Journal International Du Cancer·Y MiyoshiS Noguchi
Aug 15, 2002·The Journal of Cell Biology·Thomas A KuferErich A Nigg
Oct 23, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Tomotoshi MarumotoHideyuki Saya
Mar 29, 2005·Nature Genetics·Xiaochun YuJunjie Chen

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Citations

Aug 20, 2009·The Journal of Clinical Investigation·Zheng FuDonald J Tindall
Oct 7, 2014·Molecular Carcinogenesis·Longfeng LuYoujun Li
Oct 1, 2015·The Journal of Biological Chemistry·Yingying GuoMichael S Y Huen

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