Generation and characterization of recombinant bivalent fusion protein r-Cpib for immunotherapy against Clostridium perfringens beta and iota toxemia

Molecular Immunology
Shreya DasHarsh V Batra

Abstract

Clostridium perfringens beta (CPB) and iota (CPI) toxaemias result in some of the most lethal forms of haemorrhagic and necrotic enteritis and sudden death syndrome affecting especially neonates. While CPB enterotoxemia is one of the most common forms of clostridial enterotoxemia, CPI enterotoxemia though putatively considered to be rare is an emerging cause of concern. The similarities in clinical manifestation, gross and histopathology findings of both types of toxaemias coupled to the infrequency of CPI toxaemia might lead to symptomatic misidentification with Type C resulting in therapeutic failure due to habitual administration of CPB anti-toxin which is ineffective against CPI. Therefore in the present study, to generate a composite anti-toxin capable of neutralizing both toxaemias, a novel bivalent chimera r-Cpib was constructed by splicing the non-toxic C terminal binding regions of CPB and CPI, via a flexible glycine linker (G4S) by overlap-extension PCR. The fusion protein was characterized for its therapeutic abilities toward CPI and CPB toxin neutralizations. The r-Cpib was found to be non-toxic and could competitively inhibit binding of CPB to host cell receptors thereby reducing its cytotoxicity. Immunization of m...Continue Reading

References

Jan 1, 1987·Toxicon : Official Journal of the International Society on Toxinology·J Sakurai, Y Fujii
Apr 1, 1994·Vaccine·J D HuntD J Stewart
Jul 9, 1999·FEMS Immunology and Medical Microbiology·S Springer, H J Selbitz
Oct 12, 2000·Clinical Microbiology Reviews·M A Keller, E R Stiehm
Jul 10, 2003·The Journal of Biological Chemistry·Masahiro NagahamaJun Sakurai
May 8, 2008·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Francisco A Uzal, J Glenn Songer
Jul 16, 2008·Infection and Immunity·Jorge E VidalFrancisco A Uzal

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Apr 24, 2021·Tropical Animal Health and Production·Lida Abdolmohammadi Khiav, Azadeh Zahmatkesh

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