PMID: 8938716Jan 1, 1996Paper

Generation and characterization of subtype-specific monoclonal antibodies to K+ channel alpha- and beta-subunit polypeptides

Neuropharmacology
Z Bekele-ArcuriJ S Trimmer

Abstract

Molecular characterization of mammalian voltage-sensitive K+ channel genes and their expression became possible with the cloning of the Shaker locus of Drosophila. However, analysis of the expression patterns and subunit composition of native K+ channel protein complexes requires immunological probes specific for the individual K+ channel gene products expressed in excitable tissue. Here, we describe the generation and characterization of monoclonal antibodies (mAbs) against eight distinct mammalian K+ channel polypeptides; the Kv1.1, Kv1.2, Kv1.4, Kv1.5 and Kv1.6 Shaker-related alpha-subunits, the Kv2.1 Shab-related alpha-subunit, and the Kv beta 1 and Kv beta 2 beta-subunits. We characterized the subtype-specificity of these mAbs against native K+ channels in mammalian brain and against recombinant K+ channels expressed in transfected mammalian cells. In addition, we used these mAbs to investigate the cellular and subcellular distribution of the corresponding polypeptides in rat cerebral cortex, as well as their expression levels across brain regions.

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Citations

Dec 19, 2003·Neuropharmacology·Michael DruzinStaffan Johansson
Apr 12, 2003·Neuropharmacology·M WinklhoferS Saaler-Reinhardt
Jun 1, 1997·Brain Research. Molecular Brain Research·S Verma-KurvariR H Joho
Nov 24, 2011·Journal of Proteome Research·Min-Young SongKang-Sik Park
Nov 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·M N RasbandJ S Trimmer
Nov 8, 2001·Proceedings of the National Academy of Sciences of the United States of America·L N ManganasJ S Trimmer
Apr 16, 2008·The Journal of General Physiology·Kenneth J Rhodes, James S Trimmer
Dec 23, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Helene VacherJames S Trimmer
Feb 24, 2004·Annual Review of Physiology·James S Trimmer, Kenneth J Rhodes
Nov 15, 2011·American Journal of Physiology. Cell Physiology·Bula J BhattacharyyaRichard J Miller
Aug 8, 2008·Journal of Neurophysiology·Ricardo H PinedaAngeles B Ribera
Nov 19, 2003·BMC Neuroscience·Laurence GoutebrozeJean-Antoine Girault
Nov 25, 2005·BMC Neuroscience·Ann-Sophie PerssonCatharina Lavebratt
Dec 19, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Mark N MillerSacha B Nelson
Dec 26, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ido HorreshElior Peles
May 30, 2008·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Yasuhiro OgawaMatthew N Rasband
Oct 22, 2003·Proceedings of the National Academy of Sciences of the United States of America·Olga SokolovaNikolaus Grigorieff
Dec 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Jae-Won YangJames S Trimmer
Sep 10, 2003·The Journal of Cell Biology·Sebastian PoliakElior Peles
Mar 2, 2011·The Journal of Cell Biology·Hélène VacherJames S Trimmer
Nov 20, 2002·The Journal of Cell Biology·Matthew N RasbandJames S Trimmer
Dec 15, 2004·Critical Reviews in Biochemistry and Molecular Biology·Hiroaki Misonou, James S Trimmer
Jul 24, 2004·The Journal of Physiology·Judith BrouilletteCéline Fiset
Sep 5, 2002·The Journal of Comparative Neurology·Hon-Kit WongNikolaus J Sucher
Aug 25, 2004·Biochemical and Biophysical Research Communications·Louis N Manganas, James S Trimmer
Jan 11, 2005·Journal of Neuroscience Research·Bernd SokolowskiDmytro Duzhyy
Mar 1, 2005·The Journal of Comparative Neurology·Brian W StrassleJames S Trimmer
Nov 19, 2003·Journal of Neurochemistry·Krishna MenonSteven E Pfeiffer
Jan 22, 2009·Journal of Neuroscience Research·Yasuhiro Ogawa, Matthew N Rasband
Nov 18, 2006·The Journal of Comparative Neurology·Audra Van WartGary Matthews
Jan 24, 2007·The European Journal of Neuroscience·Jacqueline RiveraDon B Arnold
Apr 12, 2014·Journal of Neuroscience Research·Martial A DufourJean-Marc Goaillard
May 25, 2005·Journal of Neuroscience Methods·Hiroaki Misonou, James S Trimmer

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