Generation and characterization of urokinase receptor-deficient mice

The Journal of Clinical Investigation
M DewerchinD Collen

Abstract

Mice homozygously deficient for the urokinase-type plasminogen activator (u-PA) receptor (u-PAR-1-) were generated by homologous recombination in D3, embryonic stem cells. The genomic sequences comprising exon 2 through 5 of the u-PAR gene were replaced by the neomycin resistance gene, resulting in inactivation of both u-PAR splice variants. The inactivated u-PAR allele was transmitted via mendelian inheritance, and fertility. Inactivation of u-PAR was confirmed by the absence of binding of rabbit anti-murine u-PAR or of an aminoterminal fragment of murine u-PA (mu-PA.1-48) to u-PAR-1- embryonic fibroblasts and macrophages. u-PAR-1- mice displayed normal lysis of a murine plasma clot injected via the jugular vein. Invasion of macrophages into the peritoneal cavity after thioglycollate stimulation was similar in u-PAR-1- and u-PAR-1- mice. u-PAR-1- peritoneal macrophages had a threefold decreased initial rate of u-PA-mediated plasminogen activation in vitro but degraded extracellular matrix proteins in vitro as efficiently as u-PAR-1- macrophages.

References

Mar 11, 1991·The Journal of Clinical Investigation·A R Nusrat, H A Chapman
Apr 1, 1987·The Journal of Cell Biology·F BlasiK Danø
Apr 1, 1974·The Journal of Experimental Medicine·J C UnkelessE Reich
Sep 15, 1994·European Journal of Biochemistry·H R LijnenD Collen
Aug 25, 1995·The Journal of Biological Chemistry·L UmansH Van den Berghe

❮ Previous
Next ❯

Citations

Jul 3, 1997·International Journal of Cancer. Journal International Du Cancer·P A AndreasenM J Duffy
Jan 1, 1996·International Journal of Clinical & Laboratory Research·H R Lijnen
May 17, 2011·Cellular and Molecular Neurobiology·Nicholas SeedsKenneth Minor
May 2, 2006·Clinical & Experimental Metastasis·Crispin R DassPeter F M Choong
Dec 25, 2012·Molecular Neurobiology·Xavier Ekolle Ndode-Ekane, Asla Pitkänen
Jan 8, 1999·Molecular and Biochemical Parasitology·P J RosenthalE F Plow
Dec 18, 2001·FEMS Microbiology Reviews·K LähteenmäkiT K Korhonen
Sep 9, 1999·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·M R Del BigioM Altumbabic
Jan 11, 2001·Journal of Pharmacological and Toxicological Methods·R J LeadleyA Gagnon
Dec 28, 1999·Microbes and Infection·N Khalil
Jul 30, 2002·European Journal of Haematology·Pierre F Piguet, Christian Vesin
Dec 18, 2007·Nature Medicine·Changli WeiJochen Reiser
Jun 2, 1998·Kidney International·P Carmeliet, D Collen
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·T H BuggeJ L Degen
Sep 22, 1998·The Journal of Experimental Medicine·A E MayK T Preissner
Jan 31, 2012·Cardiovascular Research·Revu Ann AlexanderJohannes M Breuss
Jun 23, 2000·Journal of Pediatric Hematology/oncology·J S Palumbo, J L Degen
Nov 1, 1996·European Journal of Biochemistry·H R LijnenD Collen
Mar 1, 2000·American Journal of Respiratory Cell and Molecular Biology·D A WaltzH A Chapman
May 4, 2011·Vascular Cell·Rashna D BalsaraVictoria A Ploplis
May 23, 2009·PLoS Pathogens·Joppe W R HoviusTom van der Poll
Feb 26, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Gregory B BissonetteElizabeth M Powell
Dec 17, 2015·Expert Opinion on Therapeutic Targets·Shih-Chi SuShun-Fa Yang
Sep 6, 2007·The Journal of Cell Biology·Mònica SuelvesPura Muñoz-Cánoves
Dec 1, 2001·Expert Opinion on Biological Therapy·B MuehlenwegN Harbeck
Dec 25, 2002·The Journal of Cell Biology·Zhong MaSteven L Gonias
Mar 3, 1997·The Journal of Experimental Medicine·A R KitchingP G Tipping
Oct 9, 2004·International Journal of Cancer. Journal International Du Cancer·Kasper AlmholtMorten Johnsen
Jun 13, 2008·The Journal of Investigative Dermatology·Yosuke KannoHiroyuki Matsuno

❮ Previous
Next ❯

Related Concepts

Related Feeds

Blood Clotting Disorders

Thrombophilia includes conditions with increased tendency for excessive blood clotting. Blood clotting occurs when the body has insufficient amounts of specialized proteins that make blood clot and stop bleeding. Here is the latest research on blood clotting disorders.