Generation and functional characterization of MDSC-like cells

Oncoimmunology
Annkristin HeinePeter Brossart

Abstract

Myeloid-derived suppressor cells (MDSC) are critical in regulating immune responses by suppressing antigen presenting cells (APC) and T cells. We previously observed that incubation of peripheral blood monocytes with interleukin (IL)-10 during their differentiation to monocyte-derived dendritic cells (moDCs) results in the generation of an APC population with a CD14+HLA-DRlowphenotype (IL-10-APC) with reduced stimulatory capacity similar to human MDSC. Co-incubation experiments now revealed that the addition of IL-10-APC to moDC caused a reduction of DC-induced T-cell proliferation, of the expression of maturation markers, and of secreted cytokines and chemokines such as TNF-α, IL-6, MIP-1α and Rantes. Addition of IL-10-APC increased the immunosuppressive molecule osteoactivin and its corresponding receptor syndecan-4 on moDC. Moreover, CD14+HLA-DRlow MDSC isolated from healthy donors expressed high levels of osteoactivin, which was even further upregulated by the auxiliary addition of IL-10. Using transcriptome analysis, we identified a set of molecules and pathways mediating these effects. In addition, we found that IL-10-APC as well as human isolated MDSC expressed higher levels of programmed death (PD)-1, PD-ligand-1 (PD-L1...Continue Reading

References

Jun 3, 2000·Annual Review of Immunology·J BanchereauK Palucka
Dec 18, 2001·Journal of Cellular Biochemistry·F F SafadiS N Popoff
Mar 16, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Katrin M BaltzHelmut R Salih
Aug 24, 2007·Advances in Experimental Medicine and Biology·Srinivas Nagaraj, Dmitry I Gabrilovich
Oct 20, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jin-Sung ChungKiyoshi Ariizumi
Apr 17, 2008·Cancer Research·Srinivas Nagaraj, Dmitry I Gabrilovich
Dec 4, 2008·FEMS Immunology and Medical Microbiology·Myung-Chul ChungSerguei G Popov
Oct 2, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jin-Sung ChungKiyoshi Ariizumi
Oct 3, 2009·Nature Protocols·Athanasios TheocharidisTom C Freeman
Feb 23, 2010·Current Opinion in Immunology·Elisa PeranzoniVincenzo Bronte
May 26, 2010·Nature Reviews. Immunology·Christian KurtsPercy A Knolle
May 27, 2010·Journal of Leukocyte Biology·Thomas CondamineElise Chiffoleau
Aug 30, 2011·Cancer Immunology, Immunotherapy : CII·Mark-Alexander SchwarzbichFrank Grünebach
Oct 18, 2012·The Journal of Biological Chemistry·Bhumika SharmaLeonidas C Platanias
Oct 25, 2012·Anti-cancer Agents in Medicinal Chemistry·Sabrin H Albeituni, Jun Yan
Feb 12, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jin-Sung ChungKiyoshi Ariizumi
May 3, 2014·Seminars in Oncology·C Marcela Diaz-MonteroAlberto J Montero
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Mar 10, 2015·Nature Methods·Daehwan KimSteven L Salzberg
May 13, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jessica HoppstädterAlexandra K Kiemer
Dec 19, 2015·Nature Immunology·Florent GinhouxSubhra K Biswas
May 19, 2016·Immunity·Margaret K CallahanJedd D Wolchok

❮ Previous
Next ❯

Citations

Dec 7, 2017·Oncoimmunology·Abhishek D GargLorenzo Galluzzi
Sep 3, 2020·Frontiers of Medicine·Mahmoud Mohammad YaseenAmmar Daoud
Apr 20, 2018·Frontiers in Immunology·Hiroyuki HoriguchiUNKNOWN Sepsis and Critical Illness Research Center Investigators
Jun 4, 2019·Frontiers in Immunology·Graham PawelecSuzanne Ostrand-Rosenberg
Oct 28, 2019·Oncoimmunology·Jenny SprootenAbhishek D Garg
May 3, 2020·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Peter Brossart
Sep 1, 2018·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Mario P Colombo
Feb 8, 2021·Cellular Immunology·Graham PawelecSuzanne Ostrand-Rosenberg
Apr 27, 2021·Journal of Autoimmunity·Glenn F van WigcherenGeorgina Flórez-Grau
Jun 3, 2021·Cells·Mithunah KrishnamoorthySaman Maleki Vareki
Oct 19, 2021·Frontiers in Physiology·Kyu Hwan KwackKeith L Kirkwood
Nov 9, 2021·Frontiers in Immunology·Harry RobertsonNatasha M Rogers

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE92852

Methods Mentioned

BETA
PCA
nuclear translocation
RNA-Seq
FACS
enzyme-linked immunosorbent assay
PCR

Software Mentioned

PGS
DESeq2
GOEA
CASAVA
Prism
Partek Genomics Suite
CENA
HISAT

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.