PMID: 9555891Apr 29, 1998Paper

Generation and properties of a Streptococcus pneumoniae mutant which does not require choline or analogs for growth

Journal of Bacteriology
J YotherW Fischer

Abstract

A mutant (JY2190) of Streptococcus pneumoniae Rx1 which had acquired the ability to grow in the absence of choline and analogs was isolated. Lipoteichoic acid (LTA) and wall teichoic acid (TA) isolated from the mutant were free of phosphocholine and other phosphorylated amino alcohols. Both polymers showed an unaltered chain structure and, in the case of LTA, an unchanged glycolipid anchor. The cell wall composition was also not altered except that, due to the lack of phosphocholine, the phosphate content of cell walls was half that of the parent strain. Isolated cell walls of the mutant were resistant to hydrolysis by pneumococcal autolysin (N-acetylmuramyl-L-alanine amidase) but were cleaved by the muramidases CPL and cellosyl. The lack of active autolysin in the mutant cells became apparent by impaired cell separation at the end of cell division and by resistance against stationary-phase and penicillin-induced lysis. As a result of the absence of choline in the LTA, pneumococcal surface protein A (PspA) was no longer retained on the cytoplasmic membrane. During growth in the presence of choline, which was incorporated as phosphocholine into LTA and TA, the mutant cells separated normally, did not release PspA, and became pen...Continue Reading

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Citations

May 20, 2003·European Journal of Biochemistry·Niklas BergströmUffe B Skov Sørensen
Nov 2, 2005·Protein Science : a Publication of the Protein Society·Laura LagarteraMargarita Menéndez
Dec 14, 2007·Journal of Lipid Research·Michael Schlame
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Nov 13, 2004·FEMS Microbiology Reviews·Rubens López, Ernesto García
Mar 22, 2012·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Dalia DenapaiteWaldemar Vollmer
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Oct 26, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Emilie Vassal-StermannNicole M Thielens
May 13, 2017·Proceedings of the National Academy of Sciences of the United States of America·Thomas R Larson, Janet Yother
Jun 8, 2019·Microbiology Spectrum·Waldemar VollmerAlexander Tomasz

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