Abstract
In developing countries, bacterial acute gastroenteritis continues to be an important cause of morbidity and mortality among young children. Salmonellosis constitutes a major cause of infectious enteritis worldwide, most of them associated to the consumption of contaminated food products. Traditionally, Salmonella has been classified in serovars based on varieties of O and H surface antigens. In the present work we generated and characterized a panel of anti-flagellin monoclonal antibodies (MAbs) in order to select antibodies useful for detecting the H surface antigen. Four different MAbs were obtained by somatic hybridization of splenocytes. We found two MAbs that recognised regions of flagellin conserved among different Salmonella serovars. Other two MAbs recognised structures restricted to Salmonella enterica sv. Typhimurium, being one of them suitable for agglutination tests. Using a diverse panel of S. enterica serovars with different H antigen varieties we confirmed that this MAb agglutinates specifically S. Typhimurium (antigenic formula: 4,12:i:1,2) or other serovars expressing flagellar factor i. In conclusion, we generated a valuable immunochemical tool to be used in simple assays for serotyping of epidemiologically r...Continue Reading
References
Jan 1, 1985·Medical Microbiology and Immunology·G F IbrahimR A Walker
Jan 1, 1996·Molecular Microbiology·S I Aizawa
Aug 6, 1999·Journal of Pediatric Gastroenterology and Nutrition·F G ChirdoC A Fossati
Oct 8, 1999·Emerging Infectious Diseases·P S MeadR V Tauxe
Jul 18, 2000·Molecular Pathology : MP·P N NelsonP G Murray
Dec 19, 2000·Veterinary Microbiology·M SojkaM J Woodward
Jun 8, 2002·FEMS Immunology and Medical Microbiology·Ianko D IankovIvan G Mitov
Oct 19, 2004·Trends in Microbiology·Hugo Cruz RamosJean-Claude Sirard
Nov 18, 2006·Journal of Molecular Biology·Raghu Ram V MalapakaBrian C Tripp
Sep 22, 2007·Foodborne Pathogens and Disease·Steven L FoleyRobert D Walker
Jan 7, 2009·Applied and Environmental Microbiology·A RementeriaJ Garaizar
Feb 18, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Shannon E MajowiczUNKNOWN International Collaboration on Enteric Disease 'Burden of Illness' Studies
May 21, 2010·Journal of Clinical Microbiology·L BetancorJ A Chabalgoity
Apr 7, 2011·European Journal of Immunology·Saeeda BobatAdam F Cunningham
Apr 16, 2011·Foodborne Pathogens and Disease·Rene S HendriksenFrank M Aarestrup
Jul 19, 2012·World Journal of Microbiology & Biotechnology·Yanina HiriartMartin Rumbo
Citations
May 30, 2015·Immunologic Research·Chandresh SharmaAshutosh Tiwari
Mar 22, 2016·PloS One·Girish RamachandranRaphael Simon
Aug 16, 2019·AMB Express·Yi YangGuoqiang Zhu
Mar 4, 2017·Protein Science : a Publication of the Protein Society·Y HiriartM Rumbo
Sep 24, 2019·Antibodies·Devendra BhandariRoger C Bridgman