Generation and Validation of a Complete Knockout Model of abcc6a in Zebrafish

The Journal of Investigative Dermatology
M Van GilsO M Vanakker

Abstract

Pseudoxanthoma elasticum is an ectopic mineralization disease due to biallelic ABCC6 mutations. As no treatment options are currently available, a reliable zebrafish model is invaluable for high throughput compound screening. However, data from previously reported knockdown and mutant zebrafish models for abcc6a, the functional orthologue of ABCC6, showed phenotypic discrepancies. To address this, we developed a complete abcc6a knockout model using Clustered Regularly Interspaced Short Palindromic Repeats/Cas9 and compared its phenotype to that of a mutant model (Sa963) and a splice junction morpholino model. Our data showed that abcc6a is not required for embryonic survival, but rather that it has an essential role in controlling mineralization. The three models developed very similar hypermineralization of spine and ribs starting embryonically and progressing in adulthood with development of scoliosis. Our results indicate a direct relation between loss of abcc6a expression and dysregulated osteogenesis. As such, our models recapitulate part of the human phenotype in which ectopic mineralization and pro-osteogenic signaling have been reported. Because of its reproducibility in three models and its ease of quantification, we c...Continue Reading

Citations

Sep 3, 2020·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Shana VerschuereOlivier M Vanakker
Jan 2, 2021·International Journal of Molecular Sciences·Jianjian SunTao P Zhong
Nov 2, 2020·FEBS Letters·Shana VerschuereOlivier M Vanakker
Mar 27, 2021·Frontiers in Cell and Developmental Biology·Dávid CzimerMáté Varga
May 1, 2021·International Journal of Molecular Sciences·Briana K ShimadaOlivier Le Saux

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