Generation and Validation of miR-142 Knock Out Mice

PloS One
Amit ShresthaS Bellusci

Abstract

microRNA-142 (miR-142) is an important regulator of many biological processes and associated signaling pathways during embryonic development, homeostasis and disease. The miR-142 hairpin gives rise to the "guide strand" miR-142-3p and the sister "passenger" strand miR-142-5p. miR-142-3p has been shown to play critical, non-redundant functions in the development of the hematopoietic lineage. We have recently reported that miR-142-3p is critical for the control of Wnt signaling in the mesenchyme of the developing lung. miR-142-5p has been proposed to control adaptive growth in cardiomyocytes postnatally and its increase is associated with extensive apoptosis and cardiac dysfunction in a murine heart failure model. Using homologous recombination, we now report the generation and validation of miR-142-null mice. miR-142-null mice show a significant decrease in th expression levels of both the 3p and 5p isoforms. The expression of Bzrap1, a gene immediately flanking miR-142 is not altered while the expression of a long non-coding RNA embedded within the miR-142 gene is decreased. miR-142-null newborn pups appear normal and are normally represented indicating absence of embryonic lethality. At embryonic day 18.5, miR-142-null lungs d...Continue Reading

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Citations

Dec 30, 2015·Journal of Clinical Medicine·Yohei ShimonoHironobu Minami
May 4, 2016·Cellular and Molecular Life Sciences : CMLS·Yuanyuan Wei, Andreas Schober
May 25, 2016·Molecular and Cellular Pediatrics·Claudio Nardiello, Rory E Morty
May 5, 2017·European Journal of Immunology·Alexander MildnerSteffen Jung
Nov 25, 2016·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Amit ShresthaSaverio Bellusci
May 8, 2018·Viruses·Yongxiu YaoVenugopal Nair
Mar 20, 2019·Cellular and Molecular Life Sciences : CMLS·Amit ShresthaSaverio Bellusci

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Methods Mentioned

BETA
ubiquitination
PCR
genotyping
gene trap
gene-trap
pull-down

Software Mentioned

Targetscan
MicroT
mirPath
Graph Pad Prism Pad
Diana

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