Generation, maintenance and reactivity of ovine T-lymphocyte clones derived from sheep immunized with pili from Bacteroides nodosus.

Veterinary Immunology and Immunopathology
D L EmeryJ A Maclaren

Abstract

Peripheral blood leucocytes (PBL) from sheep immunized with pilus protein purified from Bacteroides nodosus serogroup A were cultivated in vitro and cloned in the presence of the specific antigen and autologous antigen-presenting cells (APC). The efficiency of cloning was enhanced by high proliferative responses to pili during the initial week of cultivation, and the provision of recombinant human interleukin-2 (rec-IL-2). After three passages at weekly intervals, bulk cultures of PBL and cloned T-lymphocytes were greater than 99% CD4+, CD8-, sIg-, i.e. the characteristic phenotype of helper T-lymphocytes. Cloned T-lymphocytes were devoid of allo-reactivity, and were restricted by class II antigens of the major histocompatibility complex (MHC). Both bulk PBL and cloned T-lymphocytes exhibited similar patterns of reactivity against pili from different serogroups of B. nodosus and the T-lymphocytes reacted to three of six peptides synthesized from the amino-acid sequence of pilus from serogroup A. Although clones of T-lymphocytes could retain antigen specificity for up to 2 months of cultivation, several attempts to recover clones with specific reactivity after storage in liquid nitrogen were unsuccessful.

References

Jan 1, 1987·Annual Review of Immunology·A M Livingstone, C G Fathman
Jan 1, 1986·European Journal of Immunology·C R MackayM R Brandon
Nov 1, 1987·Veterinary Immunology and Immunopathology·D L EmeryM R Brandon
Oct 1, 1984·Veterinary Immunology and Immunopathology·G Oldham, L Williams

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Citations

Apr 1, 1994·Vaccine·J D HuntD J Stewart
Jul 1, 1996·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·R Whittington
Oct 1, 1993·Immunology and Cell Biology·T J O'MearaH W Raadsma

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