Generation of a bioengineered tooth by using a three-dimensional cell manipulation method (organ germ method)

Methods in Molecular Biology
Masamitsu OshimaTakashi Tsuji

Abstract

The arrangement of cells within a tissue plays an essential role in organogenesis, including tooth development. Organ morphogenesis and physiological functions induced by three-dimensional tissue organization are well known to be regulated by the proper spatiotemporal organization of various signaling molecules, including cytokines, extracellular matrix proteins, and adhesion molecules. Development of a three-dimensional cell manipulation technology to create a bioengineered organ germ, designated as the organ germ method, enabled the generation of a structurally correct and fully functional bioengineered tooth in vivo. This method is expected to be utilized as a valuable technique for analyzing gene and protein functions during organogenesis. Here, we describe protocols for tooth germ reconstitution using the organ germ method and methods for analyzing tooth development in vitro and in vivo.

Citations

Oct 3, 2013·Nature Communications·Masatoshi HirayamaTakashi Tsuji
Jan 7, 2015·Regenerative Medicine·Thimios A Mitsiadis, Hidemitsu Harada
May 3, 2014·Scientific Reports·Sara Yajima-HimuroMatsuo Yamamoto
May 7, 2016·Journal of Tissue Engineering and Regenerative Medicine·Nelson Monteiro, Pamela C Yelick
Jan 9, 2021·Ecotoxicology and Environmental Safety·Qingqing WengYing Zhang
Sep 30, 2021·Tissue Engineering. Part C, Methods·Huijiao YanYoshizo Matsuka

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