Abstract
HIV-derived vectors are of potential clinical relevance due to their ability to transduce nondividing cells in vitro and in vivo. However, the generation of cell lines stably and reproducibly expressing high amounts of defined subviral particles, capable of packaging and transducing HIV-derived vectors, has been hampered by the cytotoxicity of some of the required gene products, in particular of the HIV-1 protease. The successful use of regulatable gene expression systems to overcome this problem requires that the remaining basally expressed gene product activity is below the threshold for cytotoxicity. To try to achieve this, we have consecutively introduced appropriate plasmids, encoding HIV rev and HIV gag/pol gene products, each under the control of separate ecdysone-inducible promoters, into human 293 cells. Using a protocol in which a specific HIV protease inhibitor, Saquinavir, was continuously present in the culture medium during selection, we could generate stable cell lines inducibly expressing high amounts of subviral particles. A cell line, termed 293-Rev/Gag/Pol(i), which has been characterized in more detail, inducibly releases, within 48 h postinduction, high amounts of HIV Gag/Pol particles (about 10 microg CA/m...Continue Reading
References
Mar 25, 1992·Nucleic Acids Research·R A MorganW F Anderson
Nov 1, 1992·Journal of Virology·B ChesebroS Perryman
Jan 1, 1992·Journal of Virology·H G Kräusslich
Jan 1, 1992·Virology·K MergenerH G Kräusslich
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R L ShoemanM C Graves
Aug 1, 1990·Molecular and Cellular Biology·D G MillerA D Miller
Jan 24, 1985·Nature·L RatnerK Baumeister
Sep 1, 1989·Journal of Virology·A LeverJ Sodroski
Aug 1, 1986·Journal of Virology·A AdachiM A Martin
Sep 2, 1988·Science·K StrebelM A Martin
Jun 1, 1995·Journal of Virology·H SchaalA Scheid
Apr 1, 1995·Journal of Virology·J CleverT G Parslow
Mar 1, 1995·The Journal of General Virology·J H RichardsonA M Lever
Feb 25, 1995·Nucleic Acids Research·Y SoneokaA J Kingsman
Nov 4, 1994·Cell·M M RollsJ K Rose
Dec 2, 1993·Nature·T P YaoR M Evans
Feb 1, 1993·Virology·H G KräusslichV Bosch
Feb 28, 1993·Gene·H SchaalA Scheid
Apr 12, 1996·Science·L NaldiniD Trono
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·D NoR M Evans
Jul 1, 1996·Journal of Virology·H YuJ P Dougherty
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·P R StrackB D Korant
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·P CorbeauF Wong-Staal
Apr 1, 1997·Trends in Biochemical Sciences·P A WadeA P Wolffe
Jun 1, 1997·Journal of Virology·M S McBrideA T Panganiban
Aug 1, 1997·Journal of Virology·N SrinivasakumarD Rekosh
Feb 7, 1998·Science·R N De GuzmanM F Summers
Sep 19, 1998·Virology·M KaulJ P Dougherty
Oct 10, 1998·Journal of Virology·T DullL Naldini
Nov 13, 1998·Journal of Virology·R ZuffereyD Trono
Dec 16, 1998·Journal of Virology·T KafriI M Verma
Feb 11, 1999·Journal of Virology·M GasmiS T Chen
May 14, 1999·Current Opinion in Genetics & Development·S Y Archer, R A Hodin
Mar 4, 2000·Human Gene Therapy·T SchnellK Uberla
Jun 14, 2000·Archives of Biochemistry and Biophysics·J SnáselI Pichová
Aug 19, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·N KlagesD Trono
Citations
Mar 6, 2009·Gene Therapy·H J StewartP A Radcliffe
Jan 9, 2008·Molecular Therapy : the Journal of the American Society of Gene Therapy·Sophie BroussauBernard Massie
Apr 30, 2003·Human Gene Therapy·Cordula LeursHelmut Hanenberg
Feb 8, 2003·DNA and Cell Biology·Ricardo Quinonez, Richard E Sutton
Jun 19, 2013·Human Gene Therapy Methods·Anna StornaiuoloChiara Bovolenta
Nov 13, 2010·Human Gene Therapy·Hannah J StewartPippa A Radcliffe
Sep 22, 2011·Human Gene Therapy·Scott R WittingMadhusudan V Peshwa
Mar 17, 2009·Blood·Robert E ThromJohn T Gray
Dec 29, 2006·BMC Biotechnology·Claudia MuratoriMaurizio Federico
Apr 22, 2005·Journal of Insect Science·R Lafont, L Dinan
Mar 12, 2015·Viruses·Paola Di BonitoMaurizio Federico
Mar 9, 2011·Vaccine·A SistiguM Federico
Jul 21, 2009·The Journal of Gene Medicine·Sven AnsorgeAmine Kamen
Sep 13, 2015·Virology·Elisa OrecchiniAlessandro Michienzi
Nov 24, 2005·The FEBS Journal·Subba R PalliDavid W Potter
Jan 31, 2009·European Journal of Immunology·Claudia MuratoriMaurizio Federico
Oct 18, 2012·Cellular Microbiology·Sandra Columba Cabezas, Maurizio Federico
Oct 19, 2006·Gene·Udo ZeilfelderChristine Leib-Mösch
Jul 25, 2009·The Journal of General Virology·Claudia MuratoriMaurizio Federico
Apr 26, 2016·Molecular Therapy. Methods & Clinical Development·Otto-Wilhelm MertenChiara Bovolenta
Jan 23, 2015·Retrovirology·Michela SabbatucciLaura Fantuzzi
Jun 22, 2014·Blood·Anais Girard-GagnepainEls Verhoeyen
Oct 27, 2012·Vaccine·Laura Lattanzi, Maurizio Federico
Oct 6, 2009·Virology·Paola Di BonitoMaurizio Federico
May 31, 2011·Virology·Maurizio Federico
Mar 7, 2006·Molecular Therapy : the Journal of the American Society of Gene Therapy·Adam S CockrellTal Kafri
Feb 25, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Thomas RelanderJohan Richter
Feb 5, 2005·The Journal of Gene Medicine·Yajin NiBoro Dropulic
Jan 16, 2004·Gene Therapy·B L StrangY Takeuchi
Apr 8, 2003·Nature Biotechnology·Yasuhiro IkedaMary Collins
May 6, 2017·Archives of Virology·Chiara ChiozziniMaurizio Federico
Jul 21, 2020·Biotechnology Journal·Mariana V FerreiraAna Sofia Coroadinha
May 22, 2004·Journal of Biomedical Science·Andrew M L LeverJing Zhao
Jan 1, 2020·Molecular Therapy. Methods & Clinical Development·Matthew BaulerRobert E Throm