Generation of a floxed allele of Smad5 for cre-mediated conditional knockout in the mouse

Genesis : the Journal of Genetics and Development
Lieve UmansAn Zwijsen

Abstract

Smad5 is a member of the Smad family of intracellular mediators of BMP signals and in endothelial cells of TGF-beta signals. We and others previously showed that loss of Smad5 in the mouse results in embryonic lethality (between E9.5-E11.5) due to multiple embryonic and extraembryonic defects. To circumvent the early embryonic lethality and to allow tissue- and time-specific Smad5 inactivation, we created a conditional Smad5 allele in the mouse. Floxed Smad5 (Smad5(flE2,Neo/flE2,Neo)) mice were generated in which both exon2 and the Neo-cassette were flanked by loxP sites. Here we demonstrate that embryos with ubiquitous Cre-mediated deletion of Smad5 (Smad5(flDeltaE2/flDeltaE2)) phenocopy the conventional Smad5 knockout mice. Smad5(flE2/flE2) mice are now available and will be a valuable tool to analyze the role of Smad5 beyond its crucial early embryonic function throughout development and postnatal life.

References

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Citations

Oct 10, 2015·Journal of Molecular Cell Biology·Domiziana CostamagnaMaurilio Sampaolesi
Dec 30, 2015·Stem Cell Reports·Maria Gomes FernandesSusana Chuva de Sousa Lopes
Jul 5, 2011·Journal of Theoretical Biology·Maria-Grazia AscenziGeorge Mohler
Jul 20, 2005·Genesis : the Journal of Genetics and Development·Sandra GoebbelsKlaus-Armin Nave
Dec 8, 2004·Brain Research. Molecular Brain Research·Jiangang GaoJian Zuo
Sep 24, 2015·The Journal of Cell Biology·Ine VandersmissenAernout Luttun
Feb 26, 2015·Journal of Cellular Biochemistry·Amy TascaKim C Mansky
Jun 13, 2006·Developmental Biology·Sebastian J ArnoldElizabeth J Robertson
Mar 13, 2015·Frontiers in Genetics·Jérémy ThalgottFranck Lebrin
Aug 10, 2006·Blood·Sofie SingbrantStefan Karlsson
Jan 8, 2011·American Journal of Physiology. Gastrointestinal and Liver Physiology·Joannie M AllaireNathalie Perreault

Related Concepts

Smad5 protein, mouse
Alleles
DNA Helix Destabilizing Proteins
Embryonic Structures, Mammalian
Mini-Exon
Shuttle Vectors
Genetic Carriers
Mice, Inbred C57BL
Founder Mice, Transgenic
Phosphoproteins

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