Generation of a Lineage II Powassan Virus (Deer Tick Virus) cDNA Clone: Assessment of Flaviviral Genetic Determinants of Tick and Mosquito Vector Competence

Vector Borne and Zoonotic Diseases
Joan L KenneyAaron C Brault

Abstract

The Flavivirus genus comprises a diverse group of viruses that utilize a wide range of vertebrate hosts and arthropod vectors. The genus includes viruses that are transmitted solely by mosquitoes or vertebrate hosts as well as viruses that alternate transmission between mosquitoes or ticks and vertebrates. Nevertheless, the viral genetic determinants that dictate these unique flaviviral host and vector specificities have been poorly characterized. In this report, a cDNA clone of a flavivirus that is transmitted between ticks and vertebrates (Powassan lineage II, deer tick virus [DTV]) was generated and chimeric viruses between the mosquito/vertebrate flavivirus, West Nile virus (WNV), were constructed. These chimeric viruses expressed the prM and E genes of either WNV or DTV in the heterologous nonstructural (NS) backbone. Recombinant chimeric viruses rescued from cDNAs were characterized for their capacity to grow in vertebrate and arthropod (mosquito and tick) cells as well as for in vivo vector competence in mosquitoes and ticks. Results demonstrated that the NS elements were insufficient to impart the complete mosquito or tick growth phenotypes of parental viruses; however, these NS genetic elements did contribute to a 100-...Continue Reading

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Citations

Apr 9, 2021·The Journal of Experimental Medicine·Marianna AgudeloMichel C Nussenzweig
Apr 9, 2021·The Journal of Experimental Medicine·Laura A VanBlarganMichael S Diamond
Sep 7, 2021·The Journal of General Virology·Jermilia CharlesBradley J Blitvich

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Methods Mentioned

BETA
in vitro transcription
PCR
dissection
transfection

Software Mentioned

Geneious
Prism

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