PMID: 9554535Apr 29, 1998Paper

Generation of a monoclonal antibody that inhibits the procoagulant activity of various cancer cell lines

Cancer
H InufusaM Yasutomi

Abstract

Tumor procoagulant is one of the factors responsible for disseminated intravascular coagulation and metastasis. The authors found procoagulant activity in LK52 human squamous cell carcinoma cells, which they designated cancer cell-derived blood coagulating activity 1 (CCA-1). A monoclonal antibody (MoAb) was generated to characterize this CCA-1 procoagulant activity. To date, antibodies that show an inhibitory effect on procoagulant activity as well as high reactivity in cancer cells are well known for their tissue factor specificity. Characterization of the procoagulant activity of CCA-1 was performed and an anti-CCA-1 MoAb, FS01, was generated. CCA-1 expression on the cancer cell surface was examined by flow cytometry. Procoagulant activity of various cancer cell lines and the inhibitory effect of the FS01 MoAb on this procoagulant activity was monitored by a clot timer. The enzymologic character differed from that of cancer procoagulant (CP). The FS01 MoAb inhibited the procoagulant activity of CCA-1, but did not inhibit that of tissue factor. A positive correlation was observed between the expression intensity of CCA-1 and the inhibitory effect of the FS01 MoAb on the procoagulant activity of cancer cell lines. Expression o...Continue Reading

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Citations

Dec 12, 2012·Preparative Biochemistry & Biotechnology·Alper Akkaya, Nurdan Pazarlioglu
May 8, 2009·Thrombosis Research·Katarzyna KaplinskaWojciech P Mielicki
Mar 11, 2004·Journal of Thrombosis and Haemostasis : JTH·W PickeringT W Barrowcliffe
Jul 13, 2004·Archives of Biochemistry and Biophysics·Shari RaasiWolfgang Korte

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