Generation of a Murine Model for c-MYC and BCL2 Co-expression B Cell Lymphomas

Frontiers in Oncology
Zhenming CaiXiaoming Wang

Abstract

Diffuse large B-cell lymphoma (DLBCL) is the most frequent lymphoma in adults, and is characterized as clinically and biologically heterogeneous lymphomas with diverse response to therapy and variation in clinical behavior. It's well-established that c-MYC and BCL2 play important roles in normal B-cell differentiation and tumorigenesis. B cell lymphoma with dual expression of c-MYC and BCL2 (double-expressor lymphoma, DEL) accounts for approximately one-third of DLBCL cases. DEL patients have poor outcomes after chemoimmunotherapy or autologous stem-cell transplantation. Lack of a genetic mouse tool for DEL hinders us from understanding the lymphogenesis mechanism and developing therapeutic strategies. Here, we investigated whether ectopic expression of c-MYC and BCL2 in different stages of B cells could lead to lymphoma and generate a mouse model for DEL. We observed that Co-expression of c-MYC and BCL2 in germinal center (GC) B cells, or pan-B cells could induce B cell lymphomas. The tumor-bearing mice have enlarged lymphoid organs, and B cells massively infiltrate into non-lymphoid organs including lung, liver and kidney. The tumor-bearing mice also manifested significantly shorter lifespan than the controls. In addition, ad...Continue Reading

References

Jul 28, 2010·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Christian GisselbrechtNorbert Schmitz
Mar 9, 2012·Advances in Hematology·Sabrina DonnouSylvain Fisson
Apr 3, 2012·Cell·Chi V Dang
Aug 2, 2012·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Nathalie A JohnsonRandy D Gascoyne
Aug 18, 2012·Cancer Cell·Sandrine SanderKlaus Rajewsky
Jan 22, 2013·Blood·Heike HornUNKNOWN German High-Grade Non-Hodgkin Lymphoma Study Group
Feb 11, 2014·British Journal of Haematology·Anamarija M PerryDennis D Weisenburger
Nov 11, 2014·The Journal of Clinical Investigation·Stéphanie SungaleeSandrine Roulland
Dec 31, 2016·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Alex F HerreraPhilippe Armand
Jan 11, 2017·Cancer Discovery·Jiyuan ZhangLaura Pasqualucci
Feb 6, 2017·Current Opinion in Immunology·Parham Ramezani-Rad, Robert C Rickert
Apr 6, 2018·The Journal of Experimental Medicine·Chao ChenXiaoming Wang
Apr 12, 2018·The New England Journal of Medicine·Roland SchmitzLouis M Staudt
May 23, 2018·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Wenting HuangShaoying Li
Jan 9, 2019·CA: a Cancer Journal for Clinicians·Rebecca L SiegelAhmedin Jemal

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
flow cytometry

Software Mentioned

GraphPad Prism

Related Concepts

Related Feeds

B cell Differentiation

Depending on the signal received through the B cell receptor and other receptors, B cells differentiate into follicular or marginal zone B cells. Here is the latest research pertaining to this differentiation process.

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.