PMID: 9525581Apr 3, 1998Paper

Generation of a mutant infectious bursal disease virus that does not cause bursal lesions

Journal of Virology
K YaoVikram N Vakharia

Abstract

A reverse genetics system for birnavirus, based on synthetic transcripts of the infectious bursal disease virus (IBDV) genome, was recently developed (E. Mundt and V. N. Vakharia, Proc. Natl. Acad. Sci. USA 93:11131-11136, 1996). To study the function of the 17-kDa nonstructural (NS) protein in viral growth and pathogenesis, we constructed a cDNA clone of IBDV segment A in which the first and only initiation codon (ATG) of NS protein was mutated to a stop codon (TAG). Transfection of Vero cells with combined transcripts of either modified or unmodified segment A, and with segment B, generated viable IBDV progeny. When chicken embryo fibroblast cells infected with transfectant viruses were analyzed by immunofluorescence assays using NS-specific antiserum, the mutant virus did not yield a fluorescence signal, indicating a lack of NS protein expression. Furthermore, replication kinetics and cytotoxic effects of the mutant virus were compared with those of the parental attenuated vaccine strain of IBDV (D78) in vitro. The mutant virus grew to slightly lower titers than D78 virus and exhibited decreased cytotoxic and apoptotic effects in cell culture. To evaluate the characteristics of the recovered viruses in vivo, we inoculated 3-...Continue Reading

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Citations

Mar 7, 2009·The Journal of General Virology·Laura DelguiJosé F Rodríguez
Jan 19, 2017·International Journal of Molecular Sciences·Yao Qin, Shijun J Zheng
May 15, 2013·Avian Pathology : Journal of the W.V.P.A·Vijay DurairajEgbert Mundt
Nov 5, 1999·Annual Review of Microbiology·A RoulstonP E Branton
Nov 27, 2004·Journal of Virology·Rodrigo A VillanuevaAna María Sandino
Jun 19, 2012·Archives of Virology·Hebata Allah MahgoubPete Kaiser
Jun 1, 2000·Avian Pathology : Journal of the W.V.P.A·T P Berg
Nov 26, 2010·Journal of Virology·Chitra UpadhyayVikram N Vakharia
Aug 24, 2006·Virus Genes·Daniel PetkovHolly S Sellers
Dec 20, 2005·The Journal of General Virology·Cyril Le NouënNicolas Eterradossi
Oct 10, 1998·Journal of Virology·K Yao, V N Vakharia
Mar 16, 2018·Journal of Virology·Liliana L Cubas-GaonaDolores Rodríguez

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