Generation of a New Tau Knockout (tauΔex1) Line Using CRISPR/Cas9 Genome Editing in Mice

Journal of Alzheimer's Disease : JAD
Daniel C S TanFabien Delerue

Abstract

Alzheimer's disease and other dementias present with tau pathology. Several mouse lines with knockout of the tau-encoding Mapt gene have been reported, yet findings often differed between lines and sites. Here, we report a new tau knockout strain (tauΔex1), generated by CRISPR/Cas9-mediated genome editing of intron -1/exon 1 of Mapt in C57Bl/6J mice. TauΔex1 mice had no overt phenotype, but, in line with previous models, they showed a significantly reduced susceptibility to excitotoxic seizures, with normal memory formation in young mice. This new in vivo resource will be made freely available to the research community.

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Citations

Jul 30, 2020·Acta Neuropathologica·Arne IttnerLars M Ittner
Jul 25, 2018·Journal of Alzheimer's Disease : JAD·Sudhanshu P RaikwarAsgar Zaheer
Apr 1, 2020·Frontiers in Endocrinology·Rafaella Araujo GonçalvesFernanda G De Felice
Oct 23, 2020·Neurology and Therapy·Nirmal Chandra BarmanShital Kumar Barman
Sep 19, 2019·ACS Chemical Neuroscience·Yam Nath PaudelMohd Farooq Shaikh

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