Generation of a novel transgenic mouse model for bioluminescent monitoring of survivin gene activity in vivo at various pathophysiological processes: survivin expression overlaps with stem cell markers.

The American Journal of Pathology
Fengzhi LiCarl W Porter

Abstract

Survival has been implicated to play an important role in various pathophysiological processes. However, because of a lack of appropriate animal models, the role and dynamic expression of survivin during pathophysiology are not well defined. We generated a human survivin gene promoter-driven luciferase transgenic mouse model (SPlucTg) so that dynamic survivin gene activity can be monitored during various pathophysiological conditions using in vivo imaging. Our results show that, consistent with survivin positivity in testis, luciferase activity in normal SPlucTg mice was detected in the testis of male mice. Furthermore, similar to the known requirement of transient expression of survivin for pathophysiological responses, we observed a transient luciferase expression in castrated SPlucTg male mice after supplement of androgen. Significantly, it was reported that survivin expression turns on during mouse liver injury and regeneration; a transient and dose-dependent luciferase expression in the mouse liver was observed after administration of carbon tetrachloride into SPlucTg mice. We further demonstrated that luciferase activity closely correlates with endogenous survivin expression. We also demonstrated that only a subset of cel...Continue Reading

References

Nov 24, 1999·The Biochemical Journal·F Li, D C Altieri
Nov 9, 2000·Proceedings of the National Academy of Sciences of the United States of America·D S O'ConnorD C Altieri
Nov 21, 2001·The Journal of Biological Chemistry·William H HoffmanMaureen Murphy
Aug 2, 2002·Cancer Cell·Daniel S O'ConnorDario C Altieri
Sep 11, 2002·Biochemical and Biophysical Research Communications·Masatoshi DeguchiTakeshi Nakano
Jul 30, 2003·Lancet·Paul J KimDario C Altieri
Aug 26, 2003·The American Journal of Pathology·Edward M ConwayDésiré Collen
Oct 31, 2003·Journal of Cellular Biochemistry·M A Christine PrattDawn White
Nov 12, 2003·The Journal of Pharmacology and Experimental Therapeutics·Tao ZhangBruce M Boman
Nov 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·Paola FortugnoDario C Altieri
Jan 13, 2004·Gastric Cancer : Official Journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association·Kazuhito MiyachiMasakatsu Sunagawa
Jun 25, 2004·The American Journal of Pathology·Monica IurlaroElisabetta Dejana
Oct 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·Dun YangJ Michael Bishop
Nov 3, 2004·Nature Reviews. Genetics·Dov Zipori
Dec 22, 2004·British Journal of Cancer·F Li
May 17, 2005·Cancer Cell·Janet PlesciaDario C Altieri
Aug 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Sandeep GurbuxaniJohn D Crispino
Dec 14, 2005·International Review of Cytology·Sally P Wheatley, Iain A McNeish
Feb 18, 2006·Cancer Immunology, Immunotherapy : CII·Michael J CiesielskiRobert A Fenstermaker
Mar 11, 2006·Apoptosis : an International Journal on Programmed Cell Death·M A Christine PrattL Isabel Renart
Mar 25, 2006·Journal of Cellular Physiology·Fengzhi Li, Xiang Ling
Apr 6, 2006·Cancer Research·Fang Xia, Dario C Altieri
Apr 6, 2006·The Journal of Biological Chemistry·Ningxi ZhuMuxiang Zhou
Apr 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Piyali DasguptaSrikumar Chellappan
May 30, 2006·Molecular Cancer Therapeutics·Seiji Fukuda, Louis M Pelus
Jul 4, 2006·The American Journal of Pathology·Fengzhi Li, Michael G Brattain
Aug 3, 2006·Journal of the National Cancer Institute·Boglarka GyurkoczaDario C Altieri
Oct 17, 2006·Molecular Cancer Therapeutics·Rami G AzrakYoucef M Rustum
Oct 31, 2006·Oncogene·V VairaD C Altieri
Nov 28, 2006·Frontiers in Bioscience : a Journal and Virtual Library·Allal OuhtitZakaria Yousief
Jan 5, 2007·Proceedings of the National Academy of Sciences of the United States of America·Wendy A WoodwardJeffrey M Rosen

❮ Previous
Next ❯

Citations

Feb 22, 2012·Carcinogenesis·Mako Narisawa-SaitoTohru Kiyono
May 3, 2013·The Journal of Clinical Investigation·Claus Kordes, Dieter Häussinger
Aug 14, 2010·The Biochemical Journal·Dario C Altieri
Jan 26, 2010·Journal of Experimental & Clinical Cancer Research : CR·Xiang LingFengzhi Li
Oct 23, 2020·Biotechnology Journal·Sean D MadsenKim C O'Connor

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.