Generation of a Transplantable Population of Human iPSC-Derived Retinal Ganglion Cells

Frontiers in Cell and Developmental Biology
Oriane RabesandratanaGael Orieux

Abstract

Optic neuropathies are a major cause of visual impairment due to retinal ganglion cell (RGC) degeneration. Human induced-pluripotent stem cells (iPSCs) represent a powerful tool for studying both human RGC development and RGC-related pathological mechanisms. Because RGC loss can be massive before the diagnosis of visual impairment, cell replacement is one of the most encouraging strategies. The present work describes the generation of functional RGCs from iPSCs based on innovative 3D/2D stepwise differentiation protocol. We demonstrate that targeting the cell surface marker THY1 is an effective strategy to select transplantable RGCs. By generating a fluorescent GFP reporter iPSC line to follow transplanted cells, we provide evidence that THY1-positive RGCs injected into the vitreous of mice with optic neuropathy can survive up to 1 month, intermingled with the host RGC layer. These data support the usefulness of iPSC-derived RGC exploration as a potential future therapeutic strategy for optic nerve regeneration.

References

Apr 1, 1984·Neuroscience·C J Barnstable, U C Dräger
Jun 20, 1998·The Journal of Physiology·E MarbanG F Tomaselli
Dec 25, 2008·Experimental Eye Research·Stuart J McKinnonRobert W Nickells
May 21, 2010·Investigative Ophthalmology & Visual Science·Mengfei ChenJian Ge
Apr 9, 2011·Nature·Mototsugu EirakuYoshiki Sasai
Mar 30, 2012·Biomedical Materials·Malgosia M PakulskaMolly S Shoichet
Apr 5, 2012·The Journal of Physiology·William A Catterall
Jun 19, 2012·Cell Stem Cell·Tokushige NakanoYoshiki Sasai
Sep 5, 2012·Nature Reviews. Neurology·Nancy J Newman
Jan 5, 2013·Science·Le CongFeng Zhang
Oct 26, 2013·Nature Protocols·F Ann RanFeng Zhang
Dec 10, 2013·The Journal of Comparative Neurology·Allen R RodriguezNicholas C Brecha
Feb 5, 2014·Stem Cells Translational Medicine·Hamidreza RiazifarTaosheng Huang
Apr 20, 2012·Journal of Stem Cell Research & Therapy·Matthew B JensenSu-Chun Zhang
Jun 10, 2014·Proceedings of the National Academy of Sciences of the United States of America·Sacha ReichmanOlivier Goureau
Jun 11, 2014·Nature Communications·Xiufeng ZhongM Valeria Canto-Soler
Aug 26, 2014·Seminars in Cell & Developmental Biology·Ahlem AssaliAlexandra Rebsam
Mar 15, 2015·Translational Vision Science & Technology·Remo SusannaRobert Ritch
Feb 5, 2016·Nature Communications·Praseeda VenugopalanJeffrey L Goldberg
Apr 30, 2016·The American Journal of Medicine·Antonio GrecoMarco de Vincentiis
Jul 12, 2016·Nature Neuroscience·Jung-Hwan A LimAndrew D Huberman
Aug 11, 2016·Scientific Reports·Katherine P GillRaymond C B Wong
Jun 5, 2017·Lancet·Jost B JonasSonghomitra Panda-Jonas
Jun 10, 2017·Science·Bireswar LahaAndrew D Huberman
Jul 19, 2017·Experimental Neurology·Dietmar FischerKevin K Park
Sep 21, 2017·International Journal of Molecular Sciences·Tien-Chun YangShih-Jen Chen
Oct 5, 2017·Human Molecular Genetics·Valerio CarelliAlfredo A Sadun
Oct 13, 2017·Stem Cells Translational Medicine·Valentin M SluchDonald J Zack
Jan 3, 2018·Developmental Biology·Sílvia LlonchMarius Ader

❮ Previous
Next ❯

Citations

Mar 24, 2021·Experimental Eye Research·Kevin Y Zhang, Thomas V Johnson
Jul 20, 2021·Frontiers in Cellular Neuroscience·Xuying LiXin Wei

❮ Previous
Next ❯

Methods Mentioned

BETA
magnetic-activated
electrophoresis
transfection
PCR
genotyping
enzymatic dissociation
flow cytometry
chip
chipset

Software Mentioned

ImageJ
Spike2
TreeStar
Prism
FIJI
ArrayScan
Envigo
HCS iDev Cell® studio
GraphPad
FlowJo

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Cellular and Molecular Life Sciences : CMLS
Jianyi Lyu, Xiuqian Mu
Journal of Neuro-ophthalmology : the Official Journal of the North American Neuro-Ophthalmology Society
Darcie L Moore, Jeffrey L Goldberg
© 2021 Meta ULC. All rights reserved