Generation of a tumor vaccine candidate based on conjugation of a MUC1 peptide to polyionic papillomavirus virus-like particles.

Cancer Immunology, Immunotherapy : CII
Sharmila Pejawar-GaddyIoannis Bossis

Abstract

Virus-like particles (VLPs) are promising vaccine technology due to their safety and ability to elicit strong immune responses. Chimeric VLPs can extend this technology to low immunogenicity foreign antigens. However, insertion of foreign epitopes into the sequence of self-assembling proteins can have unpredictable effects on the assembly process. We aimed to generate chimeric bovine papillomavirus (BPV) VLPs displaying a repetitive array of polyanionic docking sites on their surface. These VLPs can serve as platform for covalent coupling of polycationic fusion proteins. We generated baculoviruses expressing chimeric BPV L1 protein with insertion of a polyglutamic-cysteine residue in the BC, DE, HI loops and the H4 helix. Expression in insect cells yielded assembled VLPs only from insertion in HI loop. Insertion in DE loop and H4 helix resulted in partially formed VLPs and capsomeres, respectively. The polyanionic sites on the surface of VLPs and capsomeres were decorated with a polycationic MUC1 peptide containing a polyarginine-cysteine residue fused to 20 amino acids of the MUC1 tandem repeat through electrostatic interactions and redox-induced disulfide bond formation. MUC1-conjugated fully assembled VLPs induced robust act...Continue Reading

References

Oct 15, 1995·European Journal of Biochemistry·I BrockhausenJ Taylor-Papadimitriou
Aug 30, 1994·Proceedings of the National Academy of Sciences of the United States of America·K MiyamuraN S Young
Jul 21, 1997·Virology·M MüllerL Gissmann
May 23, 2000·Methods in Molecular Biology·S von Mensdorff-PouillyJ Hilgers
May 9, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·M P RudolfW M Kast
Aug 12, 2003·Expert Review of Vaccines·Florence BoisgéraultClaude Leclerc
Feb 21, 2004·Advances in Immunology·Anda M VladOlivera J Finn
Mar 6, 2004·Cancer Metastasis Reviews·James C Byrd, Robert S Bresalier
Aug 24, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Theodora FifisMagdalena Plebanski
Jul 12, 2005·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Uwe KarstenSteffen Goletz
Jul 20, 2005·Expert Review of Vaccines·Scott North, Charles Butts
Aug 25, 2005·Expert Review of Vaccines·Bruce Acres, Jean-Marc Limacher
Sep 26, 2006·Methods : a Companion to Methods in Enzymology·Elizabeth V L Grgacic, David A Anderson
Feb 22, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael S TurnerOlivera J Finn
May 17, 2007·Current Cancer Drug Targets·Y Li, P J Cozzi
Jun 5, 2007·Biotechnology and Bioengineering·Matthew PeaceyVernon K Ward
Mar 28, 2008·Journal of Virology·Christopher B BuckBenes L Trus
Apr 29, 2008·Human Vaccines·Polly Roy, Rob Noad
Jul 29, 2008·Current Opinion in Organ Transplantation·Stanislas Goriely, Michel Goldman
Oct 7, 2008·Cellular Immunology·Esin AktasGunnur Deniz
Oct 8, 2008·International Journal of Cancer. Journal International Du Cancer·Kalle AndreassonTorbjörn Ramqvist
Jan 22, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nina DickgreberIan F Hermans

❮ Previous
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Citations

Mar 8, 2012·Journal of the National Cancer Institute·Jeffrey Schlom
Jul 21, 2011·Journal of Drug Delivery·Aiala SalvadorJosé Luis Pedraz
Aug 10, 2013·Archives of Virology·Rui M Gil da Costa, Rui Medeiros
Apr 6, 2011·Immunotherapy·Lindzy F DodsonPeter Goedegebuure
Feb 19, 2013·Expert Review of Vaccines·Masaaki KawanoHiroshi Handa
Oct 16, 2013·Expert Review of Vaccines·Francesca UngaroLuigi Buonaguro
Jan 20, 2016·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Karin L LeeNicole F Steinmetz
Dec 19, 2013·Biotechnology and Bioengineering·Linda H L LuaAnton P J Middelberg
May 20, 2011·Current Opinion in Biotechnology·Ibrahim YildizNicole F Steinmetz
Dec 5, 2013·Human Vaccines & Immunotherapeutics·Fazren AzmiIstvan Toth
Mar 26, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Joana M SilvaHelena F Florindo
Nov 22, 2011·Research in Veterinary Science·Fuxiao LiuZhiliang Wang
Dec 17, 2014·Frontiers in Chemistry·João ConniotTeresa S Barata
Dec 12, 2018·Expert Review of Vaccines·Paul Engeroff, Martin F Bachmann
Dec 28, 2017·Nature Reviews. Immunology·Olivera J Finn
Jul 6, 2019·Frontiers in Plant Science·Ahmad IbrahimRichard Kormelink
Mar 3, 2020·Frontiers in Veterinary Science·Yuetao LiXianzhu Xia
Sep 12, 2020·Chemical Reviews·Wen-Hao Li, Yan-Mei Li

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