Generation of active oxygen in elicited cells of Arabidopsis thaliana is mediated by a NADPH oxidase-like enzyme

FEBS Letters
R DesikanS J Neill

Abstract

Suspension-cultured cells of Arabidopsis thaliana generated active oxygen species (AOS) (measured by luminol-dependent chemiluminescence) following challenge with the bacterial protein elicitor harpin or the protein kinase activator phorbol 12-myristate 13-acetate. These responses were blocked by inhibitors of superoxide dismutase (SOD), NADPH oxidase and protein kinase. Harpin treatment also resulted in an increase in cell death, a response reduced by inhibitors of AOS generation or AOS scavengers. Extracellular SOD activity was found to be present in cell culture medium. Immunoblotting of Arabidopsis extracts revealed the presence of proteins immunologically related to the human neutrophil NADPH oxidase complex, and cell-free reconstitution assays showed that human neutrophil cytosol combined with Arabidopsis membranes could initiate superoxide generation. These data suggest that the enzyme catalysing the generation of superoxide in elicited Arabidopsis cells is similar to the mammalian NADPH oxidase and that a signalling cascade leading to AOS generation involves protein phosphorylation.

References

Jun 1, 1995·Plant Physiology·J M Stone, J C Walker
Apr 25, 1995·Proceedings of the National Academy of Sciences of the United States of America·T HirayamaK Shinozaki
Jul 1, 1994·Genes & Development·K Goto, E M Meyerowitz
Jan 1, 1994·Methods in Enzymology·O T Jones, J T Hancock
May 1, 1993·Biochemical Society Transactions·O T JonesN Topley
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·S Chandra, P S Low
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·R TenhakenC Lamb
Jun 1, 1994·Plant Physiology·M. C. Mehdy

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Citations

Aug 5, 2000·Cell Biology International·E Malarczyk, M Pazdzioch-Czochra
Dec 14, 2005·Current Genetics·Cécile HervéCatherine Boyen
Jan 30, 2008·Bulletin of Experimental Biology and Medicine·V M MushtakovaV V Rogovin
Aug 10, 2006·Plant Molecular Biology·Roman A VolkovFriedrich Schöffl
Aug 5, 2000·European Journal of Pharmacology·P MoultonJ Hancock
May 29, 2003·Journal of Insect Physiology·M M.A. Whitten, N A. Ratcliffe
Mar 17, 2000·Biochimica Et Biophysica Acta·J Hernández-RuizM B Arnao
Apr 8, 2000·Free Radical Biology & Medicine·R DesikanJ T Hancock
Oct 6, 2000·Free Radical Biology & Medicine·R D JonesA H Morice
Jan 1, 1997·Free Radical Biology & Medicine·T S HiranJ T Hancock
Apr 17, 1999·The Plant Journal : for Cell and Molecular Biology·A TakahashiK Shimamoto
May 25, 2002·The Plant Journal : for Cell and Molecular Biology·Jodi A SwidzinskiChristopher J Leaver
Nov 1, 2003·Antioxidants & Redox Signaling·John R HoidalThomas P Kennedy
Feb 26, 2009·Antioxidants & Redox Signaling·Christine H Foyer, Graham Noctor
Feb 25, 2003·Journal of Experimental Botany·Fawzi A Razem, Mark A Bernards
Dec 17, 2008·Journal of Experimental Botany·L AlmagroM A Pedreño
Mar 6, 2009·Plant Physiology·Roberto De MicheleFiorella Lo Schiavo
Jan 6, 2011·Plant Physiology·Christine H Foyer, Graham Noctor
Sep 11, 1999·Plant Physiology·A K Papadakis, K A Roubelakis-Angelakis
Sep 13, 2001·Plant Physiology·R DesikanS J Neill
Oct 1, 1996·The Plant Cell·K E Hammond-Kosack, J D Jones
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Chris Lamb, Richard A. Dixon
Sep 21, 2004·Annual Review of Plant Biology·Klaus Apel, Heribert Hirt
Jul 31, 2004·Annual Review of Phytopathology·Andrew G PalmerDavid G Lynn

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