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Generation of Aggregates of Mouse ES Cells that Show Symmetry Breaking, Polarisation and Emergent Collective Behaviour in vitro

bioRxiv

May 15, 2014

Peter Baillie-JohnsonAlfonso Martinez Arias

Abstract

Dissociated mouse embryonic stem (ES) cells were cultured to form aggregates in small volumes of basal medium in U-bottomed, non tissue-culture-treated 96-well plates and subsequently maintained in suspension culture. After growth for 48 hours, the aggregates are competent to respond to...read more

Mentioned in this Paper

Adherent Culture
Bottom
Suspension Culture
PD 0325901
Formaldehyde dehydrogenase (glutathione)
Laboratory mice
Physiologic Organization
In Vitro [Publication Type]
Mouse Embryonic Stem Cells
Symmetry
9
27
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Generation of Aggregates of Mouse ES Cells that Show Symmetry Breaking, Polarisation and Emergent Collective Behaviour in vitro

bioRxiv

May 15, 2014

Peter Baillie-JohnsonAlfonso Martinez Arias

PMID: 990005215

DOI: 10.1101/005215

Abstract

Dissociated mouse embryonic stem (ES) cells were cultured to form aggregates in small volumes of basal medium in U-bottomed, non tissue-culture-treated 96-well plates and subsequently maintained in suspension culture. After growth for 48 hours, the aggregates are competent to respond to...read more

Mentioned in this Paper

Adherent Culture
Bottom
Suspension Culture
PD 0325901
Formaldehyde dehydrogenase (glutathione)
Laboratory mice
Physiologic Organization
In Vitro [Publication Type]
Mouse Embryonic Stem Cells
Symmetry
9
27

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