May 15, 2014

Generation of Aggregates of Mouse ES Cells that Show Symmetry Breaking, Polarisation and Emergent Collective Behaviour in vitro

BioRxiv : the Preprint Server for Biology
Peter Baillie-JohnsonAlfonso Martinez Arias

Abstract

Dissociated mouse embryonic stem (ES) cells were cultured to form aggregates in small volumes of basal medium in U-bottomed, non tissue-culture-treated 96-well plates and subsequently maintained in suspension culture. After growth for 48 hours, the aggregates are competent to respond to ubiquitous experimental signals which result in their symmetry-breaking and generation of defined polarised structures by 96 hours. It is envisaged that this system can be applied both to the study of early developmental events and more broadly to the processes of self-organisation and cellular decision-making. It may also provide a suitable niche for the generation of cell types present in the embryo but unobtainable from conventional adherent culture. * Chi : CHIR99021 DMH1 : Dorsomorphin-H1 ES cell : Embryonic Stem Cell PD03 : PD0325901 SB43 : SB431542

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Mentioned in this Paper

Embryo
PD 0325901
Decision Making
Entire Embryo
Adherent Culture
Suspension Culture
Chir 99021
Physiologic Organization
Mouse Embryonic Stem Cells
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