Generation of ammonia from non-urea sources in a faecal incubation system

Clinical Science and Molecular Medicine
A J VinceO M Wrong

Abstract

1. A 25% faecal suspension in sodium chloride solution, incubated anaerobically at 37 degrees C for 48 h, showed excellent survival of all the main groups of faecal bacteria. 2. All faecal incubation systems studied generated large amounts of ammonia, particularly those in which bacterial counts fell during incubation. As normal faeces contain negligible amounts of urea this ammonia must have been generated from sources other than urea. 3. Ammonia was also generated by faeces delivered by sodium chloride enema, and by ileostomy fluid, indicating that the phenomenon is not confined to distal colonic contents. 4. Ammonia generation by incubated faeces was inhibited by prior autoclaving of the sample, but not by sterilization with gamma-irradiation. 5. Generation of ammonia by incubated stool was accompanied by release of large amounts of organic anion and a fall in pH. 6. These observations are interpreted as evidence that ammonia generated within the colon in situ is not derived exclusively from urea, but also from bacterial deamination of amino acids, peptides and proteins. Simultaneously bacterial activity generates large amounts of organacid. The presence of living bacteria is not essential for ammonia generation, provided th...Continue Reading

Citations

Oct 1, 1978·Kidney International·J D Kopple
May 18, 2006·Toxicology·Jury Ju IvnitskyVladimir N Malakhovsky
Jan 1, 1984·The Proceedings of the Nutrition Society·O M Wrong, A J Vince
Apr 1, 1985·Archives of Disease in Childhood·A C DouwesJ M van der Klei-van Moorsel
Jan 1, 1987·Scandinavian Journal of Gastroenterology. Supplement·A M Stephen
Mar 1, 1988·Scandinavian Journal of Gastroenterology·H S RasmussenP B Mortensen
Jan 1, 1993·Critical Reviews in Food Science and Nutrition·M Roberfroid
Jun 13, 1998·The American Journal of Gastroenterology·B M DasaniC S Lieber
Jul 1, 1992·Scandinavian Journal of Gastroenterology·K HoltugP B Mortensen
Jan 1, 1985·Critical Reviews in Toxicology·I R RowlandA Wise

Related Concepts

Ammonia
Anaerobiosis
Administration of Enema
Feces
X-Rays, Nuclear
Gram-Negative Anaerobic Bacteria
Hydrogen-Ion Concentration
Tube Ileostomy
Streptococcus

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