PMID: 6254084Sep 1, 1980Paper

Generation of an electrochemical proton gradient in Streptococcus cremoris by lactate efflux

Proceedings of the National Academy of Sciences of the United States of America
R OttoW N Konings

Abstract

Recently an energy-recycling model was proposed that postulates the generation of an electrochemical gradient in fermentative bacteria by carrier-mediated excretion of metabolic end products in symport with protons. In this paper experimental support for this model is given. In batch cultures of Streptococcus cremoris with glucose as the sole energy source the maximal specific growth rate decreased by 30% when the external lactate concentration was decreased from 50 to 90 mM. In the same range of external lactate concentrations the molar growth yield Y for glucose as measured in energy-limited chemostat cultures also showed a 30% drop. From Y max lactose values of S. cremoris grown in the presence and absence of added lactate it was calculated that the net energy gain from the lactate efflux system was at least 12%. Lactate efflux from de-energized cells loaded with lactate could drive the uptake of leucine. This uptake was sensitive to carbonylcyanide p-trifluoromethoxyphenylhydrazone and was only partly inhibited by dicyclohexylcarbodiimide (DCCD). The limited inhibition by DCCD of lactate-induced leucine uptake indicates that ATP hydrolysis was not the driving force for transport of leucine. Uptake studies with the lipophili...Continue Reading

References

Jan 1, 1975·The Journal of Membrane Biology·P C Maloney, T H Wilson
Oct 21, 1976·Journal of Theoretical Biology·P MITCHELL
Jan 1, 1967·Biochimica Et Biophysica Acta·H A ColeD E Hughes

Citations

Sep 1, 1991·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·P Dimroth
Oct 18, 2006·Antonie van Leeuwenhoek·W N Konings
Nov 23, 1992·Biochimica Et Biophysica Acta·M GutmannR Krämer
Jan 1, 1988·The International Journal of Biochemistry·M A RimpiläinenR I Forsén
Apr 1, 1988·Biochimie·A van Boven, W N Konings
Feb 1, 1992·FEMS Microbiology Reviews·W N KoningsA J Driessen
Jan 1, 1994·Archiv für Tierernährung·J Simunek, M Marounek
Dec 1, 1982·European Journal of Biochemistry·S F BiketovV L Tsuprun
Jun 1, 1997·European Journal of Biochemistry·P D Hockings, P J Rogers
May 26, 2009·Applied and Environmental Microbiology·Maria JönssonHelge Holo
Nov 7, 2003·Applied and Environmental Microbiology·Volker Müller
May 15, 2007·Journal of Bacteriology·R J W BrooijmansJ Hugenholtz
Aug 12, 2000·Journal of Bacteriology·B J KoebmannP R Jensen
Oct 1, 1996·Antonie van Leeuwenhoek·W M de Vos
Sep 1, 1983·Antonie van Leeuwenhoek·W N Konings, R Otto
Jan 1, 1991·Antonie van Leeuwenhoek·C VerduynJohannes P van Dijken
Jan 1, 1994·Antonie van Leeuwenhoek·W N KoningsH W van Veen
Oct 3, 2008·Computational Biology and Chemistry·Ann Zahle AndersenLars Folke Olsen
Mar 7, 2013·Environmental Microbiology·Onur ErcanMichiel Kleerebezem
Sep 1, 1986·The Journal of Applied Bacteriology·H J Finlayson
Feb 11, 2015·Extremophiles : Life Under Extreme Conditions·A J Driessen, B Poolman
Jun 27, 2013·The Biochemical Journal·Gerd P BienertPascal Hols
Oct 20, 2004·Metabolic Engineering·Antonius J A van MarisJack T Pronk
Jun 15, 2015·Biochemistry. Biokhimii︠a︡·D V DibrovaA Y Mulkidjanian
Dec 13, 2003·Biochimica Et Biophysica Acta·Hiroshi Kobayashi
May 21, 2015·PloS One·Rebeca González-CabaleiroJorge Rodríguez
Apr 1, 1982·Critical Reviews in Microbiology·P N Hobson, R J Wallace
May 1, 1982·Critical Reviews in Microbiology·P N Hobson, R J Wallace
Sep 1, 1987·The Journal of Applied Bacteriology·M Marounek, S Bartos
Jan 1, 1989·Critical Reviews in Microbiology·W N KoningsA J Driessen
Jan 1, 1987·Critical Reviews in Biotechnology·J L MilnerJ M Wood
Aug 1, 1986·Biological Chemistry Hoppe-Seyler·P Dimroth, A Thomer
Nov 22, 1997·FEMS Microbiology Letters·L FutatsugiH Kobayashi
Jan 1, 1983·The Journal of Membrane Biology·H R Kaback
Jan 14, 2021·Microbial Biotechnology·Pauline L FolchRuud A Weusthuis
Jan 1, 1996·Microbiology·Stuart G Dashper, Eric C Reynolds
Mar 12, 2021·Biochemistry. Biokhimii︠a︡·M I KozlovaA Y Mulkidjanian

Related Concepts

tetraphenylphosphonium bromide
Carbonyl Cyanide p-Trifluoromethoxyphenylhydrazone
Dicyclohexylcarbodiimide
Electrophysiology (Science)
Energy Metabolism
Lactates
Leucine
Resting Potentials
Onium Compounds
Organopyrophosphorus Compounds

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Generating Insulin-Secreting Cells

Reprogramming cells or using induced pluripotent stem cells to generate insulin-secreting cells has significant therapeutic implications for diabetics. Here is the latest research on generation of insulin-secreting cells.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Epigenome Editing

Epigenome editing is the directed modification of epigenetic marks on chromatin at specified loci. This tool has many applications in research as well as in the clinic. Find the latest research on epigenome editing here.

Related Papers

Biochemistry and Molecular Biology Education : a Bimonthly Publication of the International Union of Biochemistry and Molecular Biology
Jozsef Szeberenyi
Proceedings of the National Academy of Sciences of the United States of America
A J DriessenW N Konings
© 2021 Meta ULC. All rights reserved