PMID: 39108Sep 19, 1979

Generation of anti-type III pneumococcal polysaccharide hybridomas from mice with an X-linked B-lymphocyte defect

The Journal of Experimental Medicine
K R SchroerP J Baker

Abstract

(CBA/N X BALB/c male)F1 mice bear on X-linked defect making them totally unresponsive to T-independent (TI), TI-2 antigens such as type III pneumococcal polysaccharide (SSS-III). We found that somatic cell hybrids between CB nonresponder spleen cells and NS1 plasmacytoma cells secreted antibody specific for SSS-III. The solid-phase binding of such antibody was completely inhibited by the addition of free antigen (SSS-III) and the amount of antibody detected in culture fluids ranged from 10 ng/ml to 10 micrograms/ml. Eight hybridoma clones were identified; all make antibody of the IgM class. These results indicate that the X-linked defect does not result in a deletion of a B-cell subset which responds to TI-2 antigens.

References

Oct 1, 1984·European Journal of Clinical Microbiology·R A Polin
Dec 1, 1981·In Vitro·R H Kennett
Jan 1, 1992·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·J KolbergT Steen
Feb 1, 1983·Australian and New Zealand Journal of Medicine·P J Thurlow, I F McKenzie
Jan 1, 1981·Immunological Reviews·W E GathingsM D Cooper
Dec 1, 1986·Arthritis and Rheumatism·W EmlenR P Taylor

Related Concepts

Mice, Inbred CBA
Mice, Inbred BALB C
Noonan Syndrome
B-Lymphocytes
X Chromosome
Hybrid Cells
Pneumococcal Infections
Heterotaxy, Visceral,1, X-LINKED
Antibodies, Bacterial
Ir Genes

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