Generation of bat-derived influenza viruses and their reassortants

Scientific Reports
Masahiro SatoAyato Takada

Abstract

Two novel influenza A virus-like genomes were detected in fruit bats in Central and South America. However, the biological properties of these bat-derived influenza viruses (BatIVs) are still largely unknown since infectious viral particles have never been isolated from the infected host species. In this study, a reverse genetics approach was used to generate infectious BatIV particles entirely from plasmids encoding full-length sequences in eight gene segments. We inoculated BatIV particles into various cell cultures including bat-derived cell lines and found that BatIVs infected particular bat-derived cells efficiently but not the other cell lines tested. Reassortant viruses between the two BatIVs were also successfully generated and their replication in the susceptible bat cell lines was confirmed. These findings suggest a limited host range and reassortment potential of BatIVs in nature, providing fundamental information for understanding of the ecology of BatIVs.

References

Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·G NeumannY Kawaoka
Jun 12, 2002·Biological Reviews of the Cambridge Philosophical Society·Kate E JonesNancy B Simmons
Feb 8, 2003·Proceedings of the National Academy of Sciences of the United States of America·Yutaka FujiiYoshihiro Kawaoka
Feb 15, 2005·Journal of Virology·Ron A M FouchierAlbert D M E Osterhaus
Feb 9, 2008·Emerging Infectious Diseases·Ken MaedaTetsuya Mizutani
Aug 4, 2009·PLoS Pathogens·Jonathan S TownerPierre E Rollin
Jan 18, 2011·Virology·Jeremy S Rossman, Robert A Lamb
Mar 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Suxiang TongRuben O Donis
Oct 27, 2012·Proceedings of the National Academy of Sciences of the United States of America·Adolfo García-Sastre
Dec 26, 2012·Current Opinion in Virology·Ina Smith, Lin-Fa Wang
Jan 9, 2013·Proceedings of the National Academy of Sciences of the United States of America·Xueyong ZhuIan A Wilson
Oct 17, 2013·PLoS Pathogens·Suxiang TongRuben O Donis
Oct 18, 2013·Journal of Virology·Junki MaruyamaAyato Takada
Nov 12, 2013·Emerging Infectious Diseases·Ziad A MemishW Ian Lipkin
Mar 4, 2014·Trends in Microbiology·Ying WuGeorge F Gao
Jul 24, 2014·Nature Communications·Mindaugas JuozapaitisMartin Schwemmle
Nov 14, 2014·Proceedings. Biological Sciences·Raina K PlowrightHamish McCallum
Nov 26, 2015·Virology·Junki MaruyamaAyato Takada
Sep 22, 2016·Nature Communications·Étori Aguiar MoreiraMindaugas Juozapaitis
Oct 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Étori Aguiar MoreiraGert Zimmer

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Citations

Jun 26, 2020·Frontiers in Cellular and Infection Microbiology·Efstathios S Giotis
Oct 9, 2020·Microorganisms·Yoshihiro TakadateAyato Takada
Jan 16, 2020·Cell Reports·Yoshihiro TakadateAyato Takada

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Datasets Mentioned

BETA
CY125942-CY125949

Methods Mentioned

BETA
transmission electron microscopy
electrophoresis

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