Generation of bovine (Bos indicus) and buffalo (Bubalus bubalis) adipose tissue derived stem cells: isolation, characterization, and multipotentiality

Genetics and Molecular Research : GMR
R V SampaioO M Ohashi

Abstract

Adult stem cells are known for their plasticity and their potential to differentiate into several different cell types; these characteristics have implications for cell therapy and reproductive biotechnologies. In this study, we report on the isolation and characterization of mesenchymal stem cells (MSC) derived from bovine and buffalo adipose tissue. Cells isolated using enzymatic digestion of bovine and buffalo adipose-tissue biopsy samples were grown in vitro for at least 15 passages, verifying their capacity to proliferate. These cells were also subjected to immunophenotypic characterization for the presence of CD90, CD105, and CD79, and the absence of CD45, CD34, and CD73, which are positive and negative markers of MSC, respectively. To prove their multipotency, the cells were induced to differentiate into three different cell types, chondrocytes, osteoblasts, and adipocytes, which were stained with tissue-specific dyes (Chondrogenic-Alcian Blue, Osteogenic-Alizarin Red, and Adipogenic-Oil-Red O, respectively) to confirm differentiation. Gene expression analysis of pluripotency-related genes was also conducted. Our results suggest that adipose tissue from bovines and buffalos can be used as a source of MSC, making adipose ...Continue Reading

Citations

Oct 19, 2016·Animal : an International Journal of Animal Bioscience·I LouveauF Gondret
Aug 1, 2016·Journal of Assisted Reproduction and Genetics·Moysés S MirandaOtávio M Ohashi
Jan 19, 2019·Veterinary Research Communications·Naira Caroline Godoy PieriFabiana Fernandes Bressan
Jan 27, 2019·Stem Cell Research & Therapy·Amanda Baracho Trindade HillJoaquim Mansano Garcia
Jul 15, 2020·Scientific Reports·Rafael Vilar SampaioFlávio Vieira Meirelles
Dec 2, 2017·In Vitro Cellular & Developmental Biology. Animal·Yongli YueHaiquan Yu
Dec 6, 2018·Journal of Cellular Physiology·Mudasir Bashir GugjooGutulla Taru Sharma
Oct 8, 2020·Stem Cell Reviews and Reports·Joanna BukowskaBarbara Gawrońska-Kozak

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