Generation of Cas9 transgenic zebrafish and their application in establishing an ERV-deficient animal model

Biotechnology Letters
Zhe YangHengmi Cui

Abstract

To investigate the effect of endogenous Cas9 on genome editing efficiency in transgenic zebrafish. Here we have constructed a transgenic zebrafish strain that can be screened by pigment deficiency. Compared with the traditional CRISPR injection method, the transgenic zebrafish can improve the efficiency of genome editing significantly. At the same time, we first observed that the phenotype of vertebral malformation in early embryonic development of zebrafish after ZFERV knockout. The transgenic zebrafish with expressed Cas9, is more efficient in genome editing. And the results of ZFERV knockout indicated that ERV may affect the vertebral development by Notch1/Delta D signal pathway.

References

Oct 31, 2007·Transgenic Research·Christopher T BurketDavid R Hyde
Mar 11, 2009·Journal of Visualized Experiments : JoVE·Jonathan N RosenJohn D Mably
Aug 7, 2013·Proceedings of the National Academy of Sciences of the United States of America·Li-En JaoWenbiao Chen
Mar 26, 2015·Biochemical and Biophysical Research Communications·Jian ShiGengfu Xiao
Jun 7, 2015·Genome Research·Gaurav K VarshneyShawn M Burgess
May 7, 2016·The Journal of Biological Chemistry·Sean ChenLin He
May 18, 2016·Nucleic Acids Research·Kornel LabunEivind Valen
Aug 16, 2016·Genome Research·Kejia WenGuanjun Gao

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Citations

Oct 2, 2019·Current Neuropharmacology·Angeles Edith Espino-SaldañaAtaúlfo Martínez-Torres

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Methods Mentioned

BETA
genotyping
transgenic
PCR
in vitro transcription
electrophoresis
gene knockout

Software Mentioned

Genewiz

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