Generation of cultured oligodendrocyte progenitor cells from rat neonatal brains

Current Protocols in Stem Cell Biology
Eric C LarsenI D Duncan

Abstract

The oligodendrocyte progenitor cell (OPC) is one of the most studied progenitor cells of the body. It has been extensively researched in tissue culture and more recently in vivo using a wide range of markers that recognize transcription factors and cell surface markers and identify its earliest development from neural stem cells onward. Isolation of OPCs in large numbers and in purified preparations has been sought after as a source of cells for the repair of human myelin disorders. It has been proposed that such cells could be used as an exogenous source of cells for the treatment of lesions in multiple sclerosis and the less common genetic myelin disorders such as Pelizaeus-Merzbacher disease. Prior to clinical trials, such approaches can be tested in animal models. Here, we describe the isolation of rat OPCs in culture conditions that provide large numbers of purified populations of cells.

References

Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·U TontschI D Duncan
Jan 4, 1994·Proceedings of the National Academy of Sciences of the United States of America·D A UtzschneiderI D Duncan
Sep 1, 1996·Journal of Neuroscience Research·V Avellana-AdalidA Baron-Van Evercooren
Oct 27, 1998·Journal of Neuroscience Research·S C ZhangI D Duncan
Mar 15, 2001·Journal of Neurocytology·S C ZhangI D Duncan
Jun 26, 2007·Journal of the Neurological Sciences·Ian D Duncan

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Citations

Aug 18, 2016·Current Protocols in Stem Cell Biology·Araceli Espinosa-JeffreyJean de Vellis
Nov 1, 2016·Current Protocols in Stem Cell Biology·Araceli Espinosa-JeffreyJean de Vellis

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